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GroES E.Coli/ 重组大肠杆菌GroES (HSP10)

点击次数:162发布时间:2012/10/31 20:07:55

GroES E.Coli/ 重组大肠杆菌GroES (HSP10)

更新日期:2024/10/16 16:00:43

所 在 地:美洲

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简单介绍:GroES E.Coli/ 重组大肠杆菌GroES (HSP10)

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GroES E.Coli/ 重组大肠杆菌GroES (HSP10)

产品编号:HSP-005 
产品规格:20μg/100μg/1mg

 

 

Synonyms CPN10, GROES, HSP10, HSPE1, 10 kDa chaperonin, Protein Cpn10, groES protein, 11.2 kDa stress response protein, Heat shock protein 10.
Introduction GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques.
Description Recombinant GroES produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 10.4 kDa.
Source Escherichia Coli.
Physical Appearance Sterile filtered colorless solution.
Formulation The groES protein contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 1mM DTT and 10% Glycerol.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. 
Store, frozen at -20°C for longer periods of time. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino Acid sequence MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE GEVKPLDVKVGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 

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