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GroEL E.Coli/重组大肠杆菌GroEL (HSP60)
点击次数：177发布时间：2012/10/31 20:09:53
更新日期：2024/10/16 16:00:43
所 在 地：美洲
产品型号：
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详细内容
GroEL E.Coli/重组大肠杆菌GroEL （HSP60）
产品编号：HSP-004
产品规格：20μg/100μg/1mg
产品规格：20μg/100μg/1mg
| Synonyms | CPN60, GROEL, HSP60, HSP65, SPG13, CHA60, GROL, crpA, mopA, 60 kDa chaperonin, Protein Cpn60, groEL protein, b4143, JW4103. |
| Introduction | GroEL protein is the major heat shock protein of E.coli ａnd belongs to the chaperonin （HSP60） family. GroEL protein prevents misfolding of proteins ａnd promotes the refolding ａnd proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E.coli ａnd cloned into an expression vector. This protein was overexpressed in E.coli ａnd was purified by using conventional chromatography techniques. |
| Description | Recombinant GroEL produced in E.Coli is a single, non-glycosylated polypeptide chain containing 548 amino acids ａnd having a molecular mass of 57.3kDa. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile filtered colorless solution. |
| Formulation | The groEL protein contains 25mM Tris-HCl buffer （pH 7.5）, 100mM NaCl, 1mM DTT ａnd 10% Glycerol. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino Acid Sequence | MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREIELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGIDKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDGTGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAVAKAGKPLLII AEDVEGEALA TAVVNTIRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTVISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDYDREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALIRVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNAATEEYGNmid MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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