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Dnak SBD C-terminus E.Coli/重组大肠杆菌DnaK底物结合域C末端
点击次数：179发布时间：2012/10/31 20:12:47
更新日期：2024/10/16 16:00:43
所 在 地：美洲
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Dnak SBD C-terminus E.Coli/重组大肠杆菌DnaK底物结合域C末端
产品编号：HSP-009
产品规格：20μg/100μg/1mg
产品规格：20μg/100μg/1mg
| Synonyms | HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013. |
| Introduction | DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding ａnd assembly of newly synthesized polypeptide chains ａnd in preventing the aggregation of stress-denatured proteins.The protein coding region of the substrate binding domain of DnaK （amino acids 385-638） was amplified by PCR ａnd cloned into an E. coli expression vector. The substrate binding domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid （Met） is attached at N- terminus. |
| Description | Recombinant Dnak Substrate Binding Domain C-terminal produced in E.Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids ａnd having a molecular mass of 27.7 kDa. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile filtered colorless solution. |
| Formulation | The DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT ａnd 10%Glycerol. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Sequence | MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQGERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKTAIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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