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KRT18 Human/重组人细胞角蛋白18
点击次数：177发布时间：2012/11/2 14:40:24
更新日期：2024/10/16 16:00:43
所 在 地：美洲
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KRT18 Human/重组人细胞角蛋白18
产品编号：PRO-349
产品规格：5μg/20μg/1mg
产品规格：5μg/20μg/1mg
| Synonyms | Keratin type I cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18,CYK18,Cell proliferation-inducing gene 46 protein. |
| Introduction | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. |
| Description | Cytokeratin 18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a calculated molecular mass of 48,201 Dalton, showing a 45kDa band on SDS-page, pI-5.7. The KRT18 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein （1mg/ml） was lyophilized from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM EDTA, 2mM DTT ａnd 10mM methylammonium chloride. |
| Solubility | It is recommended to reconstitute the lyophilized CK-18 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized CK-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CK-18 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Reconstitution to filaments | Performed by mixing equimolar amounts of cytokeratins of type I ａnd type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer （see above）. Protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
