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COL4A3 Human/重组类人胶原蛋白IV Alpha 3
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更新日期：2024/10/16 16:00:43
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COL4A3 Human/重组类人胶原蛋白IV Alpha 3
产品编号：PRO-387
产品规格：5μg/20μg/1mg
Synonyms Collagen alpha-3（IV） chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM. Introduction Type IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits, which are encoded by 6 different genes, alpha 1 through alpha 6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli ａnd glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 ａnd alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand. Description Human a3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" （GBM）. Recombinant antigen for solid （ELISA） ａnd fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 43,591 Dalton. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV ?3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region （situated between the N-terminal 7S domain ａnd the C-terminal noncollagenous NC1 domain）, which is a requirement for recombinant production of this antigen. Source Sf9 insect cells. Physical Appearance Sterile Filtered clear solution. Formulation The protein solution contains 20mM Hepes, pH 8.0, 80mM NaCl, 20% Glycerol. Stability Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH ａnd 20 % glycerol as cryoprotective agent. Storage temperature is -70° to -80° C. Please prevent freeze-thaw cycles. Coating concentration 0.12-0.5 ug/ml （depending on the type of ELISA plate ａnd coating buffer）. Suitable for biotinylation ａnd iodination. Applications Standard ELISA test
Immuno-Dot test with positive/negative sera panels.
SDS-PAGE
Western-Blot. Caution It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes （see Hellmark et al. in Autoantibodies, Peter, J.B. ａnd Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298）. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：5μg/20μg/1mg
| Synonyms | Collagen alpha-3（IV） chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM. |
| Introduction | Type IV collagen is a major structural component of basement membranes. It is a multimeric protein composed of 3 alpha subunits, which are encoded by 6 different genes, alpha 1 through alpha 6. Each of these alpha subunits can form a triple helix structure with 2 other subunits to form type IV collagen. The Goodpasture syndrome is a condition in which autoantibodies bind to the collagen molecules in the basement membranes of alveoli ａnd glomeruli. The epitopes that elicit these autoantibodies are restricted basically to the non-collagenous C-terminal domain of the protein. There are numerous alternative transcripts that appear to be unique to the human COL4A3 ａnd alternative splicing is limited to the six exons that encode this C-terminal domain. COL4A3 is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to the Alport syndrome are also situated within the exons that encode this C-terminal region. COL4A3 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Several exons of COL4A3 are interspersed with exons of an uncharacterized gene which is on the opposite strand. |
| Description | Human a3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" （GBM）. Recombinant antigen for solid （ELISA） ａnd fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 43,591 Dalton. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV ?3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region （situated between the N-terminal 7S domain ａnd the C-terminal noncollagenous NC1 domain）, which is a requirement for recombinant production of this antigen. |
| Source | Sf9 insect cells. |
| Physical Appearance | Sterile Filtered clear solution. |
| Formulation | The protein solution contains 20mM Hepes, pH 8.0, 80mM NaCl, 20% Glycerol. |
| Stability | Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH ａnd 20 % glycerol as cryoprotective agent. Storage temperature is -70° to -80° C. Please prevent freeze-thaw cycles. |
| Coating concentration | 0.12-0.5 ug/ml （depending on the type of ELISA plate ａnd coating buffer）. Suitable for biotinylation ａnd iodination. |
| Applications | Standard ELISA test Immuno-Dot test with positive/negative sera panels. SDS-PAGE Western-Blot. |
| Caution | It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes （see Hellmark et al. in Autoantibodies, Peter, J.B. ａnd Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298）. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
