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Collagen Human/重组类人胶原蛋白
产品编号：PRO-359
产品规格：100mg/500mg/1g
Introduction Collagen remained the initial functions of collagen ａnd had some new characters because of its structure, such as reversible colloid with heat ａnd easy processed without molecular reduction ａnd so on. However, animal collagen extracted from animal tissues was insoluble except for the molecular weight reduction.
The fetal shortcomings of animal collagen restricted the application is its harmful virus, but Human-like Collagen made up these drawbacks. Collagen （extracted from animal skin, tendons ａnd hides） is potential risk in virus with the potential to cause side effect ａnd the potential for the contamination of bovine collagen with the fatal mad cow disease. Collagen dissolves in water, which is another advantage to compete with animal collagen. It is different from hydrolyzed collagen produced by hydrolyzing animal collagen to short peptide linkage with a lower molecular weight.
Human-like collagen was expressed by human collagen gene in host, so mmunogenicity could be lessened much by compared with animal collagen after it entered into human body. Description Collagen Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain a molecular mass of 96,000 Dalton containing human collagen’s cDNA transcribed reversely from mRNA.
The Collagen is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein was lyophilized after from a sterile solution containing no additives. Solubility It is recommended to reconstitute the lyophilized Collagen in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Collagen although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Collagen should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence The sequence of the first fifteen N-terminal amino acids was determined ａnd was found to be MDPVVLQRRDWENPG. Amino acid %Asp 0.39; Thr 0.16; Ser 5.70; Pro 18.87; Gly 29.17; Ala 13.38; Val .71; Met .18; Ile 0.16; Leu 8.04; Tyr 0.90; Phe 0.15; Lys 0.27; His 0.15; Arg 0.03; Gln 17.40; Hydro Pro 4.34. Various uses of activity A. Bio-Absorption
B. Cell-Adhesion
C. Mini-immunogenicity ａnd neovascularization
D. Promoting the formation of epithelial cell
E. Biocompatibility
F. Absorption water
G. Against UV radiation.
H. Repairing defected skin
I. Removing pock, acne, spot
J. Dispelling black eye sockets, eye dropping. Biological Activity 1. Cultivation of BHK-21 ａnd 2BS cell line
BHK-21 or 2BS cells were plated onto the bottom of the 96-well cell culture plates at the density of 1*103cells/well, respectively, with 0.1ml RPMI1640 contained 10% fetal bovine serum in the well. BHK-21 or 2BS cells were incubated in standard conditions （at 37°C with 5% CO2 in a humidified incubator） for 24 hours. 2. Biochemical activity analysis of Human-like Collagen Complete culture media was removed after 24 hours, Collagen was added at various concentrations 0.01%,0.03%,0.05%,0.07%,0.09%,0.10%,0.11%,0.13% ａnd a complete medium was used as blank control. Cells were incubated in standard conditions （at 37°C with 5% CO2 in a humidified incubator）. The morphology of cell was observed by reverse microscope after 24hr, 48hr, 72hr, 96hr, ａnd 120hr.
Thereafter, the viable cell ｃount was determined by a colorimetric method using MTT （3-4,5dimethylthiazol-2yl-2, 5 diphenyl tetrazolium bromide） after 24hr, 48hr, 72hr, 96hr ａnd 120hr. A490 was evaluated by ELISA reader. 3. Results
The results demonstrate that Collagen has no significant effect on cell morphology. MTT analysis shows that Collagen has remarkable effect on cell proliferation when its concentration is higher than 0.03%. Cell proliferation was improved by 40% to 60% compared to control. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Collagen Human/重组类人胶原蛋白
产品编号：PRO-359
产品规格：100mg/500mg/1g
Introduction Collagen remained the initial functions of collagen ａnd had some new characters because of its structure, such as reversible colloid with heat ａnd easy processed without molecular reduction ａnd so on. However, animal collagen extracted from animal tissues was insoluble except for the molecular weight reduction.
The fetal shortcomings of animal collagen restricted the application is its harmful virus, but Human-like Collagen made up these drawbacks. Collagen （extracted from animal skin, tendons ａnd hides） is potential risk in virus with the potential to cause side effect ａnd the potential for the contamination of bovine collagen with the fatal mad cow disease. Collagen dissolves in water, which is another advantage to compete with animal collagen. It is different from hydrolyzed collagen produced by hydrolyzing animal collagen to short peptide linkage with a lower molecular weight.
Human-like collagen was expressed by human collagen gene in host, so mmunogenicity could be lessened much by compared with animal collagen after it entered into human body. Description Collagen Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain a molecular mass of 96,000 Dalton containing human collagen’s cDNA transcribed reversely from mRNA.
The Collagen is purified by proprietary chromatographic techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein was lyophilized after from a sterile solution containing no additives. Solubility It is recommended to reconstitute the lyophilized Collagen in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Collagen although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Collagen should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Amino acid sequence The sequence of the first fifteen N-terminal amino acids was determined ａnd was found to be MDPVVLQRRDWENPG. Amino acid %Asp 0.39; Thr 0.16; Ser 5.70; Pro 18.87; Gly 29.17; Ala 13.38; Val .71; Met .18; Ile 0.16; Leu 8.04; Tyr 0.90; Phe 0.15; Lys 0.27; His 0.15; Arg 0.03; Gln 17.40; Hydro Pro 4.34. Various uses of activity A. Bio-Absorption
B. Cell-Adhesion
C. Mini-immunogenicity ａnd neovascularization
D. Promoting the formation of epithelial cell
E. Biocompatibility
F. Absorption water
G. Against UV radiation.
H. Repairing defected skin
I. Removing pock, acne, spot
J. Dispelling black eye sockets, eye dropping. Biological Activity 1. Cultivation of BHK-21 ａnd 2BS cell line
BHK-21 or 2BS cells were plated onto the bottom of the 96-well cell culture plates at the density of 1*103cells/well, respectively, with 0.1ml RPMI1640 contained 10% fetal bovine serum in the well. BHK-21 or 2BS cells were incubated in standard conditions （at 37°C with 5% CO2 in a humidified incubator） for 24 hours. 2. Biochemical activity analysis of Human-like Collagen Complete culture media was removed after 24 hours, Collagen was added at various concentrations 0.01%,0.03%,0.05%,0.07%,0.09%,0.10%,0.11%,0.13% ａnd a complete medium was used as blank control. Cells were incubated in standard conditions （at 37°C with 5% CO2 in a humidified incubator）. The morphology of cell was observed by reverse microscope after 24hr, 48hr, 72hr, 96hr, ａnd 120hr.
Thereafter, the viable cell ｃount was determined by a colorimetric method using MTT （3-4,5dimethylthiazol-2yl-2, 5 diphenyl tetrazolium bromide） after 24hr, 48hr, 72hr, 96hr ａnd 120hr. A490 was evaluated by ELISA reader. 3. Results
The results demonstrate that Collagen has no significant effect on cell morphology. MTT analysis shows that Collagen has remarkable effect on cell proliferation when its concentration is higher than 0.03%. Cell proliferation was improved by 40% to 60% compared to control. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Collagen Human/重组类人胶原蛋白
产品编号：PRO-359
产品规格：100mg/500mg/1g
产品规格：100mg/500mg/1g
| Introduction | Collagen remained the initial functions of collagen ａnd had some new characters because of its structure, such as reversible colloid with heat ａnd easy processed without molecular reduction ａnd so on. However, animal collagen extracted from animal tissues was insoluble except for the molecular weight reduction. The fetal shortcomings of animal collagen restricted the application is its harmful virus, but Human-like Collagen made up these drawbacks. Collagen （extracted from animal skin, tendons ａnd hides） is potential risk in virus with the potential to cause side effect ａnd the potential for the contamination of bovine collagen with the fatal mad cow disease. Collagen dissolves in water, which is another advantage to compete with animal collagen. It is different from hydrolyzed collagen produced by hydrolyzing animal collagen to short peptide linkage with a lower molecular weight. Human-like collagen was expressed by human collagen gene in host, so mmunogenicity could be lessened much by compared with animal collagen after it entered into human body. |
| Description | Collagen Human Recombinant produced in E.Coli is a non-glycosylated polypeptide chain a molecular mass of 96,000 Dalton containing human collagen’s cDNA transcribed reversely from mRNA. The Collagen is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein was lyophilized after from a sterile solution containing no additives. |
| Solubility | It is recommended to reconstitute the lyophilized Collagen in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Collagen although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Collagen should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first fifteen N-terminal amino acids was determined ａnd was found to be MDPVVLQRRDWENPG. Amino acid %Asp 0.39; Thr 0.16; Ser 5.70; Pro 18.87; Gly 29.17; Ala 13.38; Val .71; Met .18; Ile 0.16; Leu 8.04; Tyr 0.90; Phe 0.15; Lys 0.27; His 0.15; Arg 0.03; Gln 17.40; Hydro Pro 4.34. |
| Various uses of activity | A. Bio-Absorption B. Cell-Adhesion C. Mini-immunogenicity ａnd neovascularization D. Promoting the formation of epithelial cell E. Biocompatibility F. Absorption water G. Against UV radiation. H. Repairing defected skin I. Removing pock, acne, spot J. Dispelling black eye sockets, eye dropping. |
| Biological Activity | 1. Cultivation of BHK-21 ａnd 2BS cell line BHK-21 or 2BS cells were plated onto the bottom of the 96-well cell culture plates at the density of 1*103cells/well, respectively, with 0.1ml RPMI1640 contained 10% fetal bovine serum in the well. BHK-21 or 2BS cells were incubated in standard conditions （at 37°C with 5% CO2 in a humidified incubator） for 24 hours. 2. Biochemical activity analysis of Human-like Collagen Complete culture media was removed after 24 hours, Collagen was added at various concentrations 0.01%,0.03%,0.05%,0.07%,0.09%,0.10%,0.11%,0.13% ａnd a complete medium was used as blank control. Cells were incubated in standard conditions （at 37°C with 5% CO2 in a humidified incubator）. The morphology of cell was observed by reverse microscope after 24hr, 48hr, 72hr, 96hr, ａnd 120hr. Thereafter, the viable cell ｃount was determined by a colorimetric method using MTT （3-4,5dimethylthiazol-2yl-2, 5 diphenyl tetrazolium bromide） after 24hr, 48hr, 72hr, 96hr ａnd 120hr. A490 was evaluated by ELISA reader. 3. Results The results demonstrate that Collagen has no significant effect on cell morphology. MTT analysis shows that Collagen has remarkable effect on cell proliferation when its concentration is higher than 0.03%. Cell proliferation was improved by 40% to 60% compared to control. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
