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CaBP28K Rat /重组大鼠钙结合蛋白D-28K
点击次数：130发布时间：2012/11/2 18:35:57
更新日期：2024/10/16 16:00:43
所 在 地：美洲
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CaBP28K Rat /重组大鼠钙结合蛋白D-28K
产品编号：PRO-400
产品规格：1μg/5μg/100μg
产品规格：1μg/5μg/100μg
| Synonyms | Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326. |
| Description | Calbindins are Ca-binding proteins belonging to the troponin C superfamily. CALB28K/Calbindin1/CALB1 （D28K/Spot35 protein or cholecalcin, rat 261 aa; mouse 261 aa; human 261-aa, chromosome 8q21.3-q22.1） was originally described as 27-kDA induced by vitamin D in the duodenum of chicken. In mammals, it is expressed in the kidney, pancreatic islets, ａnd brain. In brain, its synthesis is independent of vitamin D. CABP28K contains 4 active ａnd 2 inactive EF-hand Ca-binding domains. The gene for CABP28K is clustered in the same region as carbonic anhydrase. The neurons in the brains of patients with Huntington disease are CAB28K depleted. There are two types of CaBPs: the "trigger"- ａnd the "buffer"-CaBPs. The conformation of "trigger" type CaBPs changes upon Ca2+ binding ａnd exposes regions on protein that interact with target molecules, thus altering their activity. The buffer-type CABP are thought to control the intracellular calcium concentration. Calbindin D-28K is found predominantly in subpopulations of central ａnd peripheral nervous system neurons, ａnd in certain epithelial cells involved in Ca2+ transport such as distal tubular cells ａnd cortical collecting tubules of the kidney, ａnd in enteric neuroendocrine cells. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein was lyophilized from a concentrated solution （1mg/ml） containing 0.1mM CaCl2. |
| Solubility | It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Applications | CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay ａnd intracellular injection. For adsorption we suggest the following procedureA- Dilute 1 μl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry （final dilution 1:5＇000）. B- Add 1 μg of the recombinant protein to 1 ml of the diluted antibody solution ａnd mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections ａnd incubate for 3 days. E- Complete the immunohistochemical reaction as usual （biotinylated second antibody, ABC-complex, DAB）. As a result, the immunostaining should be strongly reduced or even completely prevented. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
