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SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
点击次数：302发布时间：2012/11/5 18:42:35
更新日期：2024/10/16 16:00:43
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SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
SARS-CoV/重组SARS冠状病毒核衣壳蛋白（1-422）
产品编号：SARS-250
产品规格：5μg/20μg/1mg
Introduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. Description The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）.
MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. Specificity The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. Purification Method Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. Source Escherichia coli. Formulation Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. Solubility Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Purity Greater than 95% as determined by SDS-PAGE. Storage Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. Applications Western blotting. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：5μg/20μg/1mg
| Introduction | SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane （M）, envelope （E）, ａnd spike （S） proteins. Spike （S）-glycoprotein of the virus interacts with a cellular receptor ａnd mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle ａnd is the primary target of neutralizing antibodies. |
| Description | The Recombinant SARS-CoV Nucleocapsid Protein is manufactured with N-terminal fusion HisTag. The Recombinant SARS-CoV Nucleocapsid His-Tagged Fusion Proteinis 47.8 kDa containing 422 amino acid residues of the SARS-CoV Nucleocapsid protein ａnd 15 additional amino acid residues – HisTag （underlined）. MRGSHHHHHH GMASHMSDNG PQSNQRSAPR ITFGGPTDST DNNQNGGRNG ARPKQRRPQG LPNNTASWFT ALTQHGKEEL RFPRGQGVPI NTNSGPDDQI GYYRRATRRV RGGDGKMKEL SPRWYFYYLG TGPEASLPYG ANKEGIVWVA TEGALNTPKD HIGTRNPNNN AATVLQLPQG TTLPKGFYAE GSRGGSQASS RSSSRSRGNS RNSTPGSSRG NSPARMASGG GETALALLLL DRLNQLESKV SGKGQQQQGQ TVTKKSAAEA SKKPRQKRTA TKQYNVTQAF GRRGPEQTQG NFGDQDLIRQ GTDYKHWPQI AQFAPSASAF FGMSRIGMEV TPSGTWLTYH GAIKLDDKDP QFKDNVILLN KHIDAYKTFP PTEPKKDKKK KTDEAQPLPQ RQKKQPTVTL LPAADMDDFS RQLQNSMSGA SADSTQA. |
| Specificity | The amino acid sequence of the recombinant SARS-CoV Nucleocapsid protein is 100% homologous to amino acid sequence of the native SARS-CoV Nucleocapsid protein. |
| Purification Method | Three-step procedure using affinity Ni-NTA chromatography ａnd size exclusion chromatography before ａnd after refolding. |
| Source | Escherichia coli. |
| Formulation | Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4. |
| Solubility | Add 0.2 ml of 0.1M Acetate buffer pH4 ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Storage | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. |
| Applications | Western blotting. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
