HIV-1 Integrase/重组艾滋病毒整合酶
产品编号：HIV-122
产品规格：100μg/500μg/1mg
产品规格：100μg/500μg/1mg
| Introduction | Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell ａnd is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain ａnd a C-terminal domain. Both Central catalytic domain ａnd C-terminal domains have been shown to bind both viral ａnd cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase ａnd may facilitate the integration process. |
| Description | The E.coli derived 26 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the pol protein （intergrase） ａnd fused with six histidines at the N-terminus. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile filtered colorless clear solution. |
| Formulation | 1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X & 50% Glycerol. |
| Purity | Greater than 95.0% as determined by HPLC analysis ａnd SDS-PAGE. |
| Stability | Protein is shipped at ambient temperature. Upon arrival, Store at -20°C Stable for Five years frozen. One month in solution at room temperature. |
| Specificity | Immunoreactive with all sera of HIV-1 infected individuals. |
| Applications | HIV-1 Integrase antigen is suitable for ELISA ａnd Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
