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HIV-1 p66 pol/重组HIV1 pol p66蛋白
产品编号：HIV-127
产品规格：2μg/10μg/100μg
Introduction Human immunodeficiency virus （HIV） is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells（specifically CD4+ T cells）, macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; ａnd thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, ａnd the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies ａnd biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active ａnd replicates, ａnd a large number of virus particles are liberated that can then infect other cells. Description HIV-1 p66 Recombinant- is a 66 kDa protein derived from pol gene. The HIV-1 p66 is glycosylated with N-linked sugars ａnd produced using baculovirus vectors in insect cells. Source Baculovirus Insect Cells. Physical Appearance Sterile filtered colorless clear solution. Formulation The HIV-1 p66 pol protein solution contains 30mM Tris pH-7, 0.15M NaCl, 0.2mM EDTA & 2mM b-ME. Purity Greater than 90.0% as determined by HPLC analysis & SDS-PAGE. Stability Recombinant HIV-1 p66 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles. Specificity Immunoreactive with sera from HIV infected individuals. Western blots : 0.1-1.0 μg/strip. ELISA : 0.05 - 0.25 μg/well. Applications HIV-1 p66 pol antigen is suitable for ELISA ａnd Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
产品规格：2μg/10μg/100μg
| Introduction | Human immunodeficiency virus （HIV） is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells（specifically CD4+ T cells）, macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; ａnd thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, ａnd the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies ａnd biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active ａnd replicates, ａnd a large number of virus particles are liberated that can then infect other cells. |
| Description | HIV-1 p66 Recombinant- is a 66 kDa protein derived from pol gene. The HIV-1 p66 is glycosylated with N-linked sugars ａnd produced using baculovirus vectors in insect cells. |
| Source | Baculovirus Insect Cells. |
| Physical Appearance | Sterile filtered colorless clear solution. |
| Formulation | The HIV-1 p66 pol protein solution contains 30mM Tris pH-7, 0.15M NaCl, 0.2mM EDTA & 2mM b-ME. |
| Purity | Greater than 90.0% as determined by HPLC analysis & SDS-PAGE. |
| Stability | Recombinant HIV-1 p66 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. |
| Specificity | Immunoreactive with sera from HIV infected individuals. |
| Western blots | : 0.1-1.0 μg/strip. |
| ELISA | : 0.05 - 0.25 μg/well. |
| Applications | HIV-1 p66 pol antigen is suitable for ELISA ａnd Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. |
