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HBV HBe/ 重组乙型肝炎病毒HBe

点击次数:207发布时间:2012/11/10 10:13:39

HBV HBe/ 重组乙型肝炎病毒HBe

更新日期:2024/10/16 16:00:55

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简单介绍:HBV HBe/ 重组乙型肝炎病毒HBe

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HBV HBe/ 重组乙型肝炎病毒HBe

产品编号:HBV-233
产品规格:100μg/500μg/1mg
 

Introduction Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
Description The E.Coli derived recombinant protein contains the HBV HBe adw immunodominant region is fused to GST tag and the Mw is 43,897.6 Dalton.
Purification Method Purified by proprietary chromatographic technique.
Purity HBV HBe protein is >95% pure as determined by 10% PAGE (coomassie staining).
Formulation 25mM Tris-HCl pH 8.0, 1.5 M urea and 50% glycerol.
Storage HBV HBe protein is shipped at ambient temperature.
Upon arrival, Store at -20°C.
Stability Five years frozen. One month in solution at room temperature.
Specificity Immunoreactive with sera HBV-infected individuals.
Applications HBV HBe antigen is suitable for ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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