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HBV X/重组乙型肝炎病毒蛋白X
点击次数：202发布时间：2012/11/10 10:14:30
更新日期：2024/10/16 16:00:55
所 在 地：美洲
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Q Q：
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
HBV X/重组乙型肝炎病毒蛋白X
产品编号：HBV-271
产品规格：5μg/25μg/1000μg
产品规格：5μg/25μg/1000μg
| Introduction | Hepatitis B virus X protein （HBx） is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation ａnd cell survival. It regulates many transcription factors including nuclear factor kappa B （NF-kappaB） ａnd plays a key role in hepatocarcinogenesis. rHBx facilitates the binding of cAMP response element binding protein （CREB） to its responsive element. rHBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK ａnd MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx ａnd general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor ａnd a negative regulator of phosphatidylinositol 3＇-kinase/AKT ａnd HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma （HCC） is involved with hepatitis B virus （HBV） infection ａnd HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC ａnd HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. |
| Description | 17 kDa protein containing 154 amino acid residues. MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNFFTSA. |
| Specificity | The amino acid sequence of the recombinant HBx is 100% homologous to amino acid sequence of the native HBx. |
| Purification Method | Two-step procedure using size exclusion chromatography before ａnd after refolding. |
| Source | Escherichia Coli. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Formulation | Sterile filtered ａnd lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH4. |
| Solubility | Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml ａnd let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. |
| Stability | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. |
| Applications | Western blotting, ELISA. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
