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HAV VP1/ 重组甲型肝炎病毒VP1
点击次数：177发布时间：2012/11/17 8:23:22
更新日期：2024/10/16 16:00:55
所 在 地：欧洲
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HAV VP1/ 重组甲型肝炎病毒VP1
产品编号：HAV-227
产品规格：100μg/500μg/1mg
产品规格：100μg/500μg/1mg
| Introduction | Forty-two antigenic domains were identified across the hepatitis A virus （HAV） polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural proteins, ａnd 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 ａnd P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth ａnd the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein ａnd the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein ａnd the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, ａnd the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein ａnd the N-terminal region of the P3D protein. Four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins. |
| Description | The E.Coli derived 48 kDa recombinant protein contains the VP1 immunodominant regions, amino acids 502-605. |
| Purification Method | HAV VP1 protein was purified by proprietary chromatographic technique. |
| Purity | HAV VP1 protein is >90% pure as determined by 10% PAGE （coomassie staining）. |
| Formulation | 10mM CBB, pH9.6, 0.1% SDS ａnd 50% glycerol. |
| Storage | HAV VP1 protein is shipped at ambient temperature. Upon arrival, Store at -20°C. |
| Stability | Five years frozen. One month in solution at room temperature. |
| Specificity | Immunoreactive with sera HAV-infected individuals. |
| Applications | HAV VP1 antigen is suitable for ELISA ａnd Western blots, excellent antigen for detection of HAV with minimal specificity problems. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
