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tPA Human/重组人组织纤溶酶原激活剂

点击次数:252发布时间:2012/11/17 8:56:26

tPA Human/重组人组织纤溶酶原激活剂

更新日期:2024/10/16 16:00:55

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简单介绍:tPA Human/重组人组织纤溶酶原激活剂

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tPA Human/重组人组织纤溶酶原激活剂

产品编号:ENZ-263
产品规格:20μg/100μg/1mg
 
 
 
Synonyms Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148.
Introduction Tissue plasminogen activator (abbreviated PLAT or tPA) is a secreted serine proteasewhich converts the proenzymeplasminogento plasmin, a fibrinolyticenzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin.
This enzyme plays a role in cell migrationand tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysiswhich can result in thrombosisor embolism.
Description Tissue Plasminogen Activator Human Recombinant produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. tPA is a serine protease enzyme that converts plasminogen to plasmin.
The tPA is purified by proprietary chromatographic techniques.
Source Chinese Hamster Ovary Cells (CHO) Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
Formulation Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.
Solubility It is recommended to reconstitute the lyophilized t-PA in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized t-PA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution tPA should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Enzymatic Activity 580,000 IU/mg.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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