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Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Lipase A/ 重组脂肪酶A
产品编号：ENZ-473
产品规格：10mg/100mg/1g
Introduction Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. Description Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. Source Escherichia Coli. Physical Appearance Sterile Filtered lyophilized powder. Formulation The protein was lyophilized without additives. Solubility It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Application Stability pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. Purity Greater than 90% as determined by SDS-PAGE. Unit Definition Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. Biological Activity 580 units/mg powder. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：10mg/100mg/1g
| Introduction | Lipase （EC 3.1.1.3） is a ubiquitous enzyme that catalyzes the hydrolysis of fats ａnd oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-（4-methoxyphynyl） glycidic acid methyl ester [（±）-MPGM] to produce （2R, 3S）-3-（4-methoxyphenyl） glycidic acid methyl ester [（-）-MPGM], an important intermediate for the synthesis of diltiazem hydrochlorid. |
| Description | Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered lyophilized powder. |
| Formulation | The protein was lyophilized without additives. |
| Solubility | It is recommended to reconstitute the lyophilized Lipase-A in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Application Stability | pH5~10 Unstable at higher than 50°C. Higher activity can be reached in 10%-50% DMSO, isopropyl ether ａnd petroleum ether, 10%-25% ethanol, acetone ａnd isopropanol, 50% activity can be kept in 50% acetone ａnd isopropanol, no activity detected in 50% ethanol. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Unit Definition | Lipase-A was assayed using p-nitrophenyl acetate （ pNPA） as a substrate. One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute. |
| Biological Activity | 580 units/mg powder. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
