产品展示
dUTPase/重组热稳定的dUTPase
点击次数：364发布时间：2012/11/17 15:17:03
更新日期：2024/10/16 16:00:55
所 在 地：欧洲
产品型号：
相关标签：dUTPase 重组热稳定的dUTPase
优质供应
详细内容
慧嘉生物您实验身边的好伙伴
为客户提供“品质的产品”和“*优质的服务”
AssayBiotech CUSABIO Immunoway Santa Abcam Cst jackson Pierce Sigma Amresco Qiagen Cayman abnova millipore invitrogen merk ebioscience prospec lifespan BD
欢迎广大客户咨询，另有大量宣传海报和小礼品赠送。
网站：www.biohj.com
Q Q：
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
Synonyms Thermostable dUTPase, dUTPase. Description Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. Source Escherichia Coli. Physical Appearance Sterile filtered liquid formulation. Formulation dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. Purity Greater than 97.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Stability Two years when stored at -20°C, 2 weeks at 4°C. Unit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Activity （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. （B） Enhancing PCR amplication : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. Specific Activity 103u/ug. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
dUTPase/重组热稳定的dUTPase
产品编号：ENZ-281
产品规格：500IU/1000IU/2000IU
产品规格：500IU/1000IU/2000IU
| Synonyms | Thermostable dUTPase, dUTPase. |
| Description | Thermostable dUTPase （pyrococcus fruriosus） maximizes the efficiency of high-fidelity PCR （using proofreading DNA polymerases）. It removes contaminating dUTP present in PCR reactions ａnd dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall ａnd slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length ａnd fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity ａnd yield-sensitive applications such as cloning ａnd subsequent recombinant protein technology, ａnd gene expression analysis （semi-quantitative RT-PCR techniques ａnd real-time PCR analysis）, where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP ａnd is critical for the fidelity of DNA replication ａnd repair. dUTPase hydrolyzes dUTP to dUMP ａnd pyrophosphate, simultaneously reducing dUTP levels ａnd providing the dUMP for dTTP biosynthesis. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile filtered liquid formulation. |
| Formulation | dUTPase is supplied in 20mM Tris-HC1 （pH 8.2）, 1mM DTT, 0.1mM EDTA, 100mM KC1, 0.1% Nonidet P40, 0.1% Tween 20 ａnd 50% glycerol at concentration of 10 u/ul of the enzyme. |
| Purity | Greater than 97.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Stability | Two years when stored at -20°C, 2 weeks at 4°C. |
| Unit Defenition | One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. |
| Activity | （A） Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepers pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. |
| （B） Enhancing PCR amplication | : 50ul of Pfu PCR reaction system with 1-3u of dUTPase （<3.0kb） or 4-6u dUTPase （>3.0kb） to amplify genomic DNA target up to 15-19 kb in length. （High concentrations of dUTPase will inhibit PCR reaction!）. |
| Specific Activity | 103u/ug. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
