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猴elisa试剂盒介绍英文说明书
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文件大小：36kb
上传时间：2012/8/20 11:15:29
文件类型：doc
上 传 者：上海泛轲实业有限公司
查看次数：263次
下载次数：55次
Purpose：
The kit is sued to assay the content of Monkey Tuberculosis Antibody in porcine serum, blood plasma, and other related Liquid samples.
Experimental principle：
The kit use ELISA to assay Monkey Tuberculosis Antibody level in the sample，use Purified Monkey Tuberculosis an antigen to coat microtitration wells, make solid-phase an antigen, add Monkey Tuberculosis Antibody in coated microtitration, Combined With HRP which labeled goat anti- Monkey antibody, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution and color develops, TMB becomes blue color At HRP enzyme-catalyzed, And at the effect of acid the color finally become yellow, the depth of color and theMonkey Tuberculosis Antibody of sample were positively correlated, measure the optical densit （OD） at 450 nm with microtiter plate reader, calculate Monkey Tuberculosis Antibody concentration by standard curve.
Materials provided with the kit
| 1 | wash solution | 20ml×1bottle | 7 | Stopp Solution | 6ml×1 bottle |
| 2 | HRP-Conjugate reagent | 6ml×1 bottle | 8 | Negative control | 0.5ml×1 bottle |
| 3 | Microelisa stripplate | 12well×8strips | 9 | Positive control | 0.5ml×1 bottle |
| 4 | Sample diluent | 6ml×1 bottle | 10 | Instruction | 1 |
| 5 | Chromogen Solution A | 6ml×1 bottle | 11 | Closure plate membrane | 2 |
| 6 | Chromogen Solution B | 6ml×1/ bottle | 12 | Sealed bags | 1 |
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