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裸鼠肌养蛋白(dystrophin)ELISA试剂盒
点击次数:174发布时间:2012/9/4 10:26:06
更新日期:2012/9/4 10:26:06
所 在 地:中国大陆
产品型号:
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详细内容
裸鼠肌养蛋白(dystrophin)ELISA试剂盒 详细资料:
编号 | 规格 | 中文名称 | 英文名称 |
FA1066B | 96T/48T | 裸鼠肌养蛋白(dystrophin)ELISA试剂盒 | Nude mouse dystrophin ELISA KIT |
实验原理:
用纯化的其抗体包被微孔板,制成固相抗体。往包被单抗的微孔中依次加入本产品,再与HRP标记的其抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中产品的浓度。
试剂盒组成:
1、30倍浓缩洗涤液 20ml×1瓶 7 终止液 6ml×1瓶
2、酶标试剂 6ml×1瓶 8 标准品(240 ng/L) 0.5ml×1瓶
3、酶标包被板 12孔×8条 9 标准品稀释液 1.5ml×1瓶
4、样品稀释液 6ml×1瓶 10 说明书 1份
5、显色剂A液 6ml×1瓶 11 封板膜 2张
6、显色剂B液 6ml×1/瓶 12 密封袋 1个
标本要求 :
1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性
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裸鼠肌养蛋白(dystrophin)ELISA试剂盒 产品优势:
1、免费代测
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4、稳定性强