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Anti-WAF1 抗体说明书
点击次数：650 发布时间：2012/9/3
Anti-WAF1 抗体说明书，报价
FROM: ABCAM
Cat. Number: Rs-0741R
Quantity size: 0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to ａnd inhibits the activity of cyclin-CDK2 or -CDK4 complexes, ａnd thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen （PCNA）, a DNA polymerase accessory factor, ａnd plays a regulatory role in S phase DNA replication ａnd DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, ａnd may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors （CKIs） have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 ａnd Cdk6 specifically.
Anti-WAF1 抗体说明书，报价
Specificity: ·anti-WAF1 is a rabbit monoclonal antibody unconjugated ·specific for WAF1 of hu, rat ａnd mo
·epitope mapping near the amino terminus of WAF1 of
hu, rat ａnd mo origin
· use for western blotting, elisa, immunoprecipitation
ａnd immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
Application: · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-WAF1 抗体说明书，报价
