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调亡诱导因子抗体信裕生物低价出售!
调亡诱导因子抗体>噬菌体抗体库技术
总体可概括为二个方面
(一)基本程序和原理 。噬菌体抗体库技术的原理,简言之就是用PCR技术从人免疫细胞中扩增出整套的抗体重链可变区(VH)和轻链可变区(VL)基因,克隆到噬菌体载体上并以融合蛋白的形式表达在其外壳表面。这样一来噬菌体DNA中有抗体基因的存在,同时在其表面又有抗体分子的表达,就可以方便地利用抗原—抗体特异性结合而筛选出所需要的抗体,并进行克隆扩增。使抗体基因以分泌的方式表达,则可获得可溶性的抗体片段。在建库过程中如果将VH和VI。随机组合,则可建成组合抗体文库;如果抗体mRNA来源于未经免疫的正常人,则可以在不需要细胞融合的情况下建立起人天然抗体库。
(二)噬菌体抗体库技术的特点,可细分为三点
1.模拟天然全套抗体库 抗体文库可以达到或超过1011库容,所以能包含B细胞全部克隆。建库的外源基因来自人体外周血,骨髓或脾脏的淋巴细胞提取的mmDNA,mmDNA反转录形成cDNA,这些mRNA是人体多克隆细胞的总mRNA。使用的通用引物采自多个人体,具有人的种属普遍性。抗体的VH和VL基因的随机重组也增加了抗体的多样性。
C96774Hu 肝细胞核因子6β(HNF6β)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 6 Beta (HNF6b) Homo sapiens (Human)
C96774Mu 肝细胞核因子6β(HNF6β)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 6 Beta (HNF6b) Mus musculus (Mouse)
C96774Ra 肝细胞核因子6β(HNF6β)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 6 Beta (HNF6b) Rattus norvegicus (Rat)
C96775Hu 肝细胞核因子1α(HNF1α)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 1 Alpha (HNF1a) Homo sapiens (Human)
C96775Mu 肝细胞核因子1α(HNF1α)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 1 Alpha (HNF1a) Mus musculus (Mouse)
C96775Ra 肝细胞核因子1α(HNF1α)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 1 Alpha (HNF1a) Rattus norvegicus (Rat)
C96776Hu 肝细胞核因子1β(HNF1β)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b) Homo sapiens (Human)
C96776Mu 肝细胞核因子1β(HNF1β)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b) Mus musculus (Mouse)
C96776Ra 肝细胞核因子1β(HNF1β)检测试剂盒(化学发光免疫分析法) CLIA Kit for Hepatocyte Nuclear Factor 1 Beta (HNF1b) Rattus norvegicus (Rat)
C95478Hu 促甲状腺素胚胎因子(TEF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Thyrotroph Embryonic Factor (TEF) Homo sapiens (Human)
C95478Mu 促甲状腺素胚胎因子(TEF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Thyrotroph Embryonic Factor (TEF) Mus musculus (Mouse)
C95478Ra 促甲状腺素胚胎因子(TEF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Thyrotroph Embryonic Factor (TEF) Rattus norvegicus (Rat)
C90101Bo 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Bos taurus; Bovine (Cattle)
C90101Ca 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Canis familiaris; Canine (Dog)
C90101Eq 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Equus caballus; Equine (Horse)
C90101Mu 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Mus musculus (Mouse)
C90101Po 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Sus scrofa; Porcine (Pig)
C90101Ra 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Rattus norvegicus (Rat)
C90101Rb 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Oryctolagus cuniculus (Rabbit)
C90102Bo 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Bos taurus; Bovine (Cattle)
C90102Cp 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Capra hircus; Caprine (Goat)
C90102Eq 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Equus caballus; Equine (Horse)
C90102Hu 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Homo sapiens (Human)
C90102Mu 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Mus musculus (Mouse)
C90102Ra 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Rattus norvegicus (Rat)
C90021Ga 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Chicken (Gallus)
C90021Hu 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Homo sapiens (Human)
C90021Mu 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Mus musculus (Mouse)
C90021Po 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Sus scrofa; Porcine (Pig)
C90021Rb 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Oryctolagus cuniculus (Rabbit)
C90024Bo 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Bos taurus; Bovine (Cattle)
C90024Hu 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Homo sapiens (Human)
C90024Mu 内分泌腺来源血管内皮生长因子(EG-VEGF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) Mus musculus (Mouse)
C90101Ca 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Canis familiaris; Canine (Dog)
C90101Eq 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Equus caballus; Equine (Horse)
C90101Mu 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Mus musculus (Mouse)
C90101Po 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Sus scrofa; Porcine (Pig)
C90101Ra 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Rattus norvegicus (Rat)
C90101Rb 基质金属蛋白酶3(MMP3)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 3 (MMP3) Oryctolagus cuniculus (Rabbit)
C90102Bo 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Bos taurus; Bovine (Cattle)
C90102Cp 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Capra hircus; Caprine (Goat)
C90102Eq 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Equus caballus; Equine (Horse)
C90102Hu 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Homo sapiens (Human)
C90102Mu 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Mus musculus (Mouse)
C90102Ra 基质金属蛋白酶7(MMP7)检测试剂盒(化学发光免疫分析法) CLIA Kit for Matrix Metalloproteinase 7 (MMP7) Rattus norvegicus (Rat)
C90021Ga 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Chicken (Gallus)
C90021Hu 睫状神经营养因子(CNTF)检测试剂盒(化学发光免疫分析法) CLIA Kit for Ciliary Neurotrophic Factor (CNTF) Homo sapiens (Human)