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溴脱氧尿苷抗体信裕生物低价出售!
溴脱氧尿苷抗体>噬菌体抗体库技术
总体可概括为二个方面
(一)基本程序和原理 。噬菌体抗体库技术的原理,简言之就是用PCR技术从人免疫细胞中扩增出整套的抗体重链可变区(VH)和轻链可变区(VL)基因,克隆到噬菌体载体上并以融合蛋白的形式表达在其外壳表面。这样一来噬菌体DNA中有抗体基因的存在,同时在其表面又有抗体分子的表达,就可以方便地利用抗原—抗体特异性结合而筛选出所需要的抗体,并进行克隆扩增。使抗体基因以分泌的方式表达,则可获得可溶性的抗体片段。在建库过程中如果将VH和VI。随机组合,则可建成组合抗体文库;如果抗体mRNA来源于未经免疫的正常人,则可以在不需要细胞融合的情况下建立起人天然抗体库。
(二)噬菌体抗体库技术的特点,可细分为三点
1.模拟天然全套抗体库 抗体文库可以达到或超过1011库容,所以能包含B细胞全部克隆。建库的外源基因来自人体外周血,骨髓或脾脏的淋巴细胞提取的mmDNA,mmDNA反转录形成cDNA,这些mRNA是人体多克隆细胞的总mRNA。使用的通用引物采自多个人体,具有人的种属普遍性。抗体的VH和VL基因的随机重组也增加了抗体的多样性。
E90878Mu 血管生成素样蛋白1(ANGPTL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Angiopoietin Like Protein 1 (ANGPTL1) Mus musculus (Mouse)
E90860Hu 上皮中性粒细胞激活肽78(ENA78)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) Homo sapiens (Human)
E90860Ra 上皮中性粒细胞激活肽78(ENA78)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) Rattus norvegicus (Rat)
E90860Mu 上皮中性粒细胞激活肽78(ENA78)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78) Mus musculus (Mouse)
E90371Hu 10kDa干扰素γ诱导蛋白(IP10)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Homo sapiens (Human)
E90371Ra 10kDa干扰素γ诱导蛋白(IP10)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Rattus norvegicus (Rat)
E90371Mu 10kDa干扰素γ诱导蛋白(IP10)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Mus musculus (Mouse)
E90374Hu GATA结合蛋白4(GATA4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for GATA Binding Protein 4 (GATA4) Homo sapiens (Human)
E90374Ra GATA结合蛋白4(GATA4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for GATA Binding Protein 4 (GATA4) Rattus norvegicus (Rat)
E90374Mu GATA结合蛋白4(GATA4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for GATA Binding Protein 4 (GATA4) Mus musculus (Mouse)
E90373Hu 结蛋白(Des)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Desmin (Des) Homo sapiens (Human)
E90373Ra 结蛋白(Des)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Desmin (Des) Rattus norvegicus (Rat)
E90373Mu 结蛋白(Des)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Desmin (Des) Mus musculus (Mouse)
E90375Hu 肌球蛋白ⅠA(MYO1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myosin IA (MYO1A) Homo sapiens (Human)
E90375Ra 肌球蛋白ⅠA(MYO1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myosin IA (MYO1A) Rattus norvegicus (Rat)
E90375Mu 肌球蛋白ⅠA(MYO1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Myosin IA (MYO1A) Mus musculus (Mouse)
E90376Hu 辅肌动蛋白α2(ACTN2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Actinin Alpha 2 (ACTN2) Homo sapiens (Human)
E91347Mu 垂体腺苷酸环化酶激活肽(ADCYAP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) Mus musculus (Mouse)
E91867Mu 表皮生长因子受体2(EGFR2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Epidermal Growth Factor Receptor 2 (EGFR2) Mus musculus (Mouse)
E91216Po 松弛肽(RLN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Relaxin (RLN) Sus scrofa; Porcine (Pig)
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E91359Pl 凝集素(PHA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phytohemagglutinin (PHA) Spinacia oleracea (Spinach)
E91896Ge 蛙皮素6-14(BB)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Bombesin 6-14 (BB) General
E90684Mu 粘蛋白5B(MUC5B)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Mucin 5 Subtype B (MUC5B) Mus musculus (Mouse)
E91266Ga 胰高血糖素(GC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glucagon (GC) Chicken (Gallus)
E90563Gu 白介素1β(IL1β)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 1 Beta (IL1b) Cavia (Guinea pig )
E90080Gu 白介素8(IL8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 8 (IL8) Cavia (Guinea pig )
E90056Gu 白介素10(IL10)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 10 (IL10) Cavia (Guinea pig )
E91980Mu 白介素33(IL33)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 33 (IL33) Mus musculus (Mouse)
E90178Po α2-HS糖蛋白(αHSG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG) Sus scrofa; Porcine (Pig)
E91154Mu 纤维蛋白原α(FGα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Alpha (FGa) Mus musculus (Mouse)
E91154Ra 纤维蛋白原α(FGα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Alpha (FGa) Rattus norvegicus (Rat)
E91154Rb 纤维蛋白原α(FGα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibrinogen Alpha (FGa) Oryctolagus cuniculus (Rabbit)