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天冬氨酸-胱氨酸特异性蛋白酶家族抗体
点击次数:198发布时间:2013/2/25 16:41:47
更新日期:2024/10/16 15:56:29
所 在 地:中国大陆
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天冬氨酸-胱氨酸特异性蛋白酶家族抗体信裕生物低价出售!
天冬氨酸-胱氨酸特异性蛋白酶家族抗体>噬菌体抗体库技术
总体可概括为二个方面
(一)基本程序和原理 。噬菌体抗体库技术的原理,简言之就是用PCR技术从人免疫细胞中扩增出整套的抗体重链可变区(VH)和轻链可变区(VL)基因,克隆到噬菌体载体上并以融合蛋白的形式表达在其外壳表面。这样一来噬菌体DNA中有抗体基因的存在,同时在其表面又有抗体分子的表达,就可以方便地利用抗原—抗体特异性结合而筛选出所需要的抗体,并进行克隆扩增。使抗体基因以分泌的方式表达,则可获得可溶性的抗体片段。在建库过程中如果将VH和VI。随机组合,则可建成组合抗体文库;如果抗体mRNA来源于未经免疫的正常人,则可以在不需要细胞融合的情况下建立起人天然抗体库。
(二)噬菌体抗体库技术的特点,可细分为三点
1.模拟天然全套抗体库 抗体文库可以达到或超过1011库容,所以能包含B细胞全部克隆。建库的外源基因来自人体外周血,骨髓或脾脏的淋巴细胞提取的mmDNA,mmDNA反转录形成cDNA,这些mRNA是人体多克隆细胞的总mRNA。使用的通用引物采自多个人体,具有人的种属普遍性。抗体的VH和VL基因的随机重组也增加了抗体的多样性。
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E92561Hu 淋巴细胞胞浆蛋白1(LCP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Lymphocyte Cytosolic Protein 1 (LCP1) Homo sapiens (Human)
E91136Mu 环胍氨酸肽(CCP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cyclical Citrullinated Peptide (CCP) Mus musculus (Mouse)
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E92024Ra 线粒体乙醛脱氢酶(ALDM)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM) Rattus norvegicus (Rat)
E92030Hu 脑型肌酸激酶(CKB)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Creatine Kinase, Brain (CKB) Homo sapiens (Human)
E90050Gu 胰岛素样生长因子1(IGF1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Insulin Like Growth Factor 1 (IGF1) Cavia (Guinea pig )
E93154Ge 维生素D4(VD4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Vitamin D4 (VD4) General
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E90523Hu 血小板衍生生长因子AA(PDGFAA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet Derived Growth Factor AA (PDGFAA) Homo sapiens (Human)
E91650Po 主要碱性蛋白(MBP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Major Basic Protein (MBP) Sus scrofa; Porcine (Pig)
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E90222Ca 干扰素β(IFNβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Beta (IFNb) Canis familiaris; Canine (Dog)
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E91589Mu 干扰素调节因子3(IRF3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Regulatory Factor 3 (IRF3) Mus musculus (Mouse)
E92450Hu 细胞外基质蛋白1(ECM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Extracellular Matrix Protein 1 (ECM1) Homo sapiens (Human)
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E90222Si 干扰素β(IFNβ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Beta (IFNb) Rhesus monkey (Simian)
E90223Ca 白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 1 Receptor Antagonist (IL1RA) Canis familiaris; Canine (Dog)
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E90224Ra 基质金属蛋白酶11(MMP11)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Matrix Metalloproteinase 11 (MMP11) Rattus norvegicus (Rat)
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