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CD3抗体信裕生物低价出售!
CD3抗体>噬菌体抗体库技术
总体可概括为二个方面
(一)基本程序和原理 。噬菌体抗体库技术的原理,简言之就是用PCR技术从人免疫细胞中扩增出整套的抗体重链可变区(VH)和轻链可变区(VL)基因,克隆到噬菌体载体上并以融合蛋白的形式表达在其外壳表面。这样一来噬菌体DNA中有抗体基因的存在,同时在其表面又有抗体分子的表达,就可以方便地利用抗原—抗体特异性结合而筛选出所需要的抗体,并进行克隆扩增。使抗体基因以分泌的方式表达,则可获得可溶性的抗体片段。在建库过程中如果将VH和VI。随机组合,则可建成组合抗体文库;如果抗体mRNA来源于未经免疫的正常人,则可以在不需要细胞融合的情况下建立起人天然抗体库。
(二)噬菌体抗体库技术的特点,可细分为三点
1.模拟天然全套抗体库 抗体文库可以达到或超过1011库容,所以能包含B细胞全部克隆。建库的外源基因来自人体外周血,骨髓或脾脏的淋巴细胞提取的mmDNA,mmDNA反转录形成cDNA,这些mRNA是人体多克隆细胞的总mRNA。使用的通用引物采自多个人体,具有人的种属普遍性。抗体的VH和VL基因的随机重组也增加了抗体的多样性。
E92130Mu 头蛋白(NOG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Noggin (NOG) Mus musculus (Mouse)
E96003Mu 神经肽FF(NPFF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neuropeptide FF (NPFF) Mus musculus (Mouse)
E97522Hu 肾连蛋白(NPNT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nephronectin (NPNT) Homo sapiens (Human)
E97819Hu 白介素1受体辅助蛋白(IL1RAP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 1 Receptor Accessory Protein (IL1RAP) Homo sapiens (Human)
E96628Hu 髓细胞触发受体2(TREM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) Homo sapiens (Human)
E96945Hu 泛素羧基端酯酶L1(UCHL1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Homo sapiens (Human)
E80604Hu HtrA丝氨酸肽酶1(HTRA1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for HtrA Serine Peptidase 1 (HTRA1) Homo sapiens (Human)
E92400Mu 肉毒碱乙酰转移酶(CRAT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carnitine Acetyltransferase (CRAT) Mus musculus (Mouse)
E97143Hu 丝氨酸肽酶抑制因子Kazal型1(SPINK1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) Homo sapiens (Human)
E92416Mu 钙蛋白酶抑制蛋白(CAST)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Calpastatin (CAST) Mus musculus (Mouse)
E94255Hu 脯氨酰4-羟化酶α多肽Ⅲ(P4Hα3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) Homo sapiens (Human)
E94762Hu 干扰素γ诱导蛋白30(IFI30)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Gamma Inducible Protein 30 (IFI30) Homo sapiens (Human)
E91242Mu ATP结合盒转运蛋白A1(ABCA1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for ATP Binding Cassette Transporter A1 (ABCA1) Mus musculus (Mouse)
E93076Mu 碳酸酐酶Ⅸ(CA9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carbonic Anhydrase IX (CA9) Mus musculus (Mouse)
E95792Mu 味觉受体1型成员2(TAS1R2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Taste Receptor Type 1 Member 2 (TAS1R2) Mus musculus (Mouse)
E94038Hu 轻肽神经丝蛋白(NEFL)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neurofilament, Light Polypeptide (NEFL) Homo sapiens (Human)
E90677Mu 凝血因子Ⅻ(F12)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Coagulation Factor XII (F12) Mus musculus (Mouse)
E90671Ra 谷胱甘肽S转移酶α3(GSTα3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glutathione S Transferase Alpha 3 (GSTa3) Rattus norvegicus (Rat)
E91582Mu IgE-Fc片段受体Ⅱ(FcεRⅡ)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin E (FceRII) Mus musculus (Mouse)
E96342Ra 色胺酸羟化酶1(TPH1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tryptophan Hydroxylase 1 (TPH1) Rattus norvegicus (Rat)
E90400Hu 视黄醇结合蛋白1(RBP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Retinol Binding Protein 1, Cellular (RBP1) Homo sapiens (Human)
E92503Mu G蛋白β1(GNβ1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for G Protein Beta 1 (GNb1) Mus musculus (Mouse)
E92972Hu 转化生长因子β受体Ⅱ(TGFβR2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Transforming Growth Factor Beta Receptor II (TGFbR2) Homo sapiens (Human)
E93198Hu 死骨片1(SQSTM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sequestosome 1 (SQSTM1) Homo sapiens (Human)
E93111Hu 胃内因子(GIF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Gastric Intrinsic Factor (GIF) Homo sapiens (Human)
E94190Hu 前蛋白转化酶枯草溶菌素1(PCSK1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) Homo sapiens (Human)
E95331Hu 细胞维甲酸结合蛋白2(CRABP2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cellular Retinoic Acid Binding Protein 2 (CRABP2) Homo sapiens (Human)
E95496Hu 驱动蛋白家族成员1A(KIF1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Kinesin Family, Member 1A (KIF1A) Homo sapiens (Human)
E92130Ra 头蛋白(NOG)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Noggin (NOG) Rattus norvegicus (Rat)
E90603Bo 载脂蛋白B100(APOB100)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Apolipoprotein B100 (APOB100) Bos taurus; Bovine (Cattle)
E91694Mu 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 25 (IL25) Mus musculus (Mouse)
E91592Ra 胱天蛋白酶1(CASP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Caspase 1 (CASP1) Rattus norvegicus (Rat)
E80154Mu 成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 15 (FGF15) Mus musculus (Mouse)
E95557Mu 线粒体解偶联蛋白1(UCP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1) Mus musculus (Mouse)
E90641Bo 分泌型免疫球蛋白A(sIgA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Secretory Immunoglobulin A (sIgA) Bos taurus; Bovine (Cattle)
E90940Po 趋化因子C-X-C-基元受体4(CXCR4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Chemokine C-X-C-Motif Receptor 4 (CXCR4) Sus scrofa; Porcine (Pig)
E80917Hu 信号素3A(SEMA3A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Semaphorin 3A (SEMA3A) Homo sapiens (Human)
E80939Hu 神经束蛋白(NFASC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neurofascin (NFASC) Homo sapiens (Human)
E92417Hu 肉毒碱棕榈酰基转移酶2(CPT2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) Homo sapiens (Human)
E94786Hu 膜联蛋白A3(ANXA3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Annexin A3 (ANXA3) Homo sapiens (Human)
E95369Hu 肉毒碱棕榈酰基转移酶1B(CPT1B)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carnitine Palmitoyltransferase 1B, Muscle (CPT1B) Homo sapiens (Human)
E95449Hu 四旋蛋白1(TSPAN1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tetraspanin 1 (TSPAN1) Homo sapiens (Human)
E99166Mu 亮氨酸丰富重复LGI家族成员3(LGI3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Leucine Rich Repeat LGI Family, Member 3 (LGI3) Mus musculus (Mouse)
E97817Hu 白介素1受体辅助蛋白样蛋白2(IL1RAPL2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) Homo sapiens (Human)
E91312Mu 分泌性白细胞蛋白酶抑制因子(SLPI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI) Mus musculus (Mouse)
E92564Hu 天冬酰胺內肽酶(LGMN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Legumain (LGMN) Homo sapiens (Human)
E97780Hu 干扰素调节因子9(IRF9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interferon Regulatory Factor 9 (IRF9) Homo sapiens (Human)
E95880Mu 分泌型卷曲相关蛋白1(SFRP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1) Mus musculus (Mouse)