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CD36抗体信裕生物低价出售!
CD36抗体>噬菌体抗体库技术
总体可概括为二个方面
(一)基本程序和原理 。噬菌体抗体库技术的原理,简言之就是用PCR技术从人免疫细胞中扩增出整套的抗体重链可变区(VH)和轻链可变区(VL)基因,克隆到噬菌体载体上并以融合蛋白的形式表达在其外壳表面。这样一来噬菌体DNA中有抗体基因的存在,同时在其表面又有抗体分子的表达,就可以方便地利用抗原—抗体特异性结合而筛选出所需要的抗体,并进行克隆扩增。使抗体基因以分泌的方式表达,则可获得可溶性的抗体片段。在建库过程中如果将VH和VI。随机组合,则可建成组合抗体文库;如果抗体mRNA来源于未经免疫的正常人,则可以在不需要细胞融合的情况下建立起人天然抗体库。
(二)噬菌体抗体库技术的特点,可细分为三点
1.模拟天然全套抗体库 抗体文库可以达到或超过1011库容,所以能包含B细胞全部克隆。建库的外源基因来自人体外周血,骨髓或脾脏的淋巴细胞提取的mmDNA,mmDNA反转录形成cDNA,这些mRNA是人体多克隆细胞的总mRNA。使用的通用引物采自多个人体,具有人的种属普遍性。抗体的VH和VL基因的随机重组也增加了抗体的多样性。
E91345Mu 四旋蛋白30(TSPAN30)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tetraspanin 30 (TSPAN30) Mus musculus (Mouse)
E93295Hu 细胞色素P450家族成员1A1(CYP1A1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cytochrome P450 1A1 (CYP1A1) Homo sapiens (Human)
E93960Hu 雌激素受体相关受体α(ERRα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Estrogen Related Receptor Alpha (ERRa) Homo sapiens (Human)
E91009Ra 极低密度脂蛋白受体(VLDLR)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Very Low Density Lipoprotein Receptor (VLDLR) Rattus norvegicus (Rat)
E91020Ra 淀粉样前体蛋白(APP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Amyloid Precursor Protein (APP) Rattus norvegicus (Rat)
E92216Hu E1A结合蛋白P300(EP300)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for E1A Binding Protein P300 (EP300) Homo sapiens (Human)
E94624Ra 周期素依赖性激酶抑制因子1A(CDKN1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Rattus norvegicus (Rat)
E92078Hu 层黏连蛋白α5(LAMα5)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Laminin Alpha 5 (LAMa5) Homo sapiens (Human)
E92172Hu 神经调节素2(NRG2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neuregulin 2 (NRG2) Homo sapiens (Human)
E93031Hu 水通道蛋白8(AQP8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Aquaporin 8 (AQP8) Homo sapiens (Human)
E95979Hu 双特异性磷酸酶9(DUSP9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dual Specificity Phosphatase 9 (DUSP9) Homo sapiens (Human)
E97552Hu 神经源性分化蛋白6(NEUROD6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neurogenic Differentiation 6 (NEUROD6) Homo sapiens (Human)
E90363Mu 血小板内皮细胞粘附分子1(PECAM1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Platelet/Endothelial Cell Adhesion Molecule (PECAM1) Mus musculus (Mouse)
E93822Mu 血小板反应蛋白2(THBS2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Thrombospondin 2 (THBS2) Mus musculus (Mouse)
E93076Ra 碳酸酐酶Ⅸ(CA9)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Carbonic Anhydrase IX (CA9) Rattus norvegicus (Rat)
E97819Mu 白介素1受体辅助蛋白(IL1RAP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Interleukin 1 Receptor Accessory Protein (IL1RAP) Mus musculus (Mouse)
E92246Mu 磷酸肌醇依赖性蛋白激酶1(PDPK1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phosphoinositide Dependent Protein Kinase 1 (PDPK1) Mus musculus (Mouse)
E92852Hu 精胺氧化酶(SMOX)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Spermine Oxidase (SMOX) Homo sapiens (Human)
E82048Hu HtrA丝氨酸肽酶4(HTRA4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for HtrA Serine Peptidase 4 (HTRA4) Homo sapiens (Human)
E90442Ra 阻抑素(PHB)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Prohibitin (PHB) Rattus norvegicus (Rat)
E90642Ra 果糖二磷酸醛缩酶A(ALDOA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Aldolase A, Fructose Bisphosphate (ALDOA) Rattus norvegicus (Rat)
E92150Mu Ⅵ型胶原α1(COL6α1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type VI Alpha 1 (COL6a1) Mus musculus (Mouse)
E93319Ra 果糖二磷酸醛缩酶B(ALDOB)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Aldolase B, Fructose Bisphosphate (ALDOB) Rattus norvegicus (Rat)
E93320Ra 果糖二磷酸醛缩酶C(ALDOC)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Aldolase C, Fructose Bisphosphate (ALDOC) Rattus norvegicus (Rat)
E93761Mu 谷氨酰胺合成酶(GS)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glutamine synthetase (GS) Mus musculus (Mouse)
E94112Hu 肝配蛋白B2(EFNB2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ephrin B2 (EFNB2) Homo sapiens (Human)
E94490Hu 溶质载体家族30成员8(SLC30A8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Solute Carrier Family 30 Member 8 (SLC30A8) Homo sapiens (Human)
E92402Hu 半胱氨酸丰富蛋白1(CRIP1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cysteine Rich Protein 1, Intestinal (CRIP1) Homo sapiens (Human)
E93761Ra 谷氨酰胺合成酶(GS)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Glutamine synthetase (GS) Rattus norvegicus (Rat)
E91845Mu CD83分子(CD83)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cluster Of Differentiation 83 (CD83) Mus musculus (Mouse)
E91276Ra 激素敏感性脂肪酶(LIPE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Lipase, Hormone Sensitive (LIPE) Rattus norvegicus (Rat)
E91448Mu 信号素7A(SEMA7A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Semaphorin 7A (SEMA7A) Mus musculus (Mouse)
E92488Hu 亚铁螯合酶(FECH)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ferrochelatase (FECH) Homo sapiens (Human)
E97574Mu N-乙酰半乳糖胺酶α(NAGα)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for N-Acetylgalactosaminidase Alpha (NAGa) Mus musculus (Mouse)
E81563Hu ⅣD组磷脂酶A2(PLA2G4D)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholipase A2, Group IVD (PLA2G4D) Homo sapiens (Human)
E90388Ca 补体成分5a(C5a)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Complement Component 5a (C5a) Canis familiaris; Canine (Dog)
E91067Mu YY肽(PYY)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Peptide YY (PYY) Mus musculus (Mouse)
E91083Ra 线粒体超氧化物歧化酶(SOD2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Superoxide Dismutase 2, Mitochondrial (SOD2) Rattus norvegicus (Rat)
E93058Ra 中性鞘磷脂酶(NSMASE)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Neutral Sphingomyelinase (NSMASE) Rattus norvegicus (Rat)
E94912Ra 沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sirtuin 1 (SIRT1) Rattus norvegicus (Rat)
E94913Ra 沉默调节蛋白3(SIRT3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Sirtuin 3 (SIRT3) Rattus norvegicus (Rat)
E97337Ra 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) Rattus norvegicus (Rat)
E91127Mu 脱氧核糖核酸酶Ⅰ(DNASE1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Deoxyribonuclease I (DNASE1) Mus musculus (Mouse)
E91929Mu 胆碱乙酰转移酶(ChAT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Choline Acetyltransferase (ChAT) Mus musculus (Mouse)
E92158Hu Ⅻ型胶原(COL12)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Collagen Type XII (COL12) Homo sapiens (Human)
E93105Hu 低密度脂蛋白受体相关蛋白6(LRP6)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Low Density Lipoprotein Receptor Related Protein 6 (LRP6) Homo sapiens (Human)
E96918Mu 尿调蛋白(UMOD)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Uromodulin (UMOD) Mus musculus (Mouse)
E94112Mu 肝配蛋白B2(EFNB2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Ephrin B2 (EFNB2) Mus musculus (Mouse)
E91085Ra 肠碱性磷酸酶(ALPI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Alkaline Phosphatase, Intestinal (ALPI) Rattus norvegicus (Rat)
E92913Hu 成纤维细胞生长因子11(FGF11)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 11 (FGF11) Homo sapiens (Human)
E92914Hu 成纤维细胞生长因子12(FGF12)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Fibroblast Growth Factor 12 (FGF12) Homo sapiens (Human)
E91166Mu VGF神经生长因子诱导蛋白(VGF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for VGF Nerve Growth Factor Inducible (VGF) Mus musculus (Mouse)
E92033Ra 热休克蛋白β8(HSPβ8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock Protein Beta 8 (HSPb8) Rattus norvegicus (Rat)