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CD44抗体信裕生物低价出售!
CD44抗体>噬菌体抗体库技术
总体可概括为二个方面
(一)基本程序和原理 。噬菌体抗体库技术的原理,简言之就是用PCR技术从人免疫细胞中扩增出整套的抗体重链可变区(VH)和轻链可变区(VL)基因,克隆到噬菌体载体上并以融合蛋白的形式表达在其外壳表面。这样一来噬菌体DNA中有抗体基因的存在,同时在其表面又有抗体分子的表达,就可以方便地利用抗原—抗体特异性结合而筛选出所需要的抗体,并进行克隆扩增。使抗体基因以分泌的方式表达,则可获得可溶性的抗体片段。在建库过程中如果将VH和VI。随机组合,则可建成组合抗体文库;如果抗体mRNA来源于未经免疫的正常人,则可以在不需要细胞融合的情况下建立起人天然抗体库。
(二)噬菌体抗体库技术的特点,可细分为三点
1.模拟天然全套抗体库 抗体文库可以达到或超过1011库容,所以能包含B细胞全部克隆。建库的外源基因来自人体外周血,骨髓或脾脏的淋巴细胞提取的mmDNA,mmDNA反转录形成cDNA,这些mRNA是人体多克隆细胞的总mRNA。使用的通用引物采自多个人体,具有人的种属普遍性。抗体的VH和VL基因的随机重组也增加了抗体的多样性。
E90872Ra 热休克蛋白40(HSP40)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock Protein 40 (HSP40) Rattus norvegicus (Rat)
E90872Mu 热休克蛋白40(HSP40)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock Protein 40 (HSP40) Mus musculus (Mouse)
E90872Fr 热休克蛋白40(HSP40)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock Protein 40 (HSP40) Xenopus (Clawed frog)
E90823Rb 90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1) Oryctolagus cuniculus (Rabbit)
E91081Po 70kDa热休克蛋白1A(HSPA1A)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A) Sus scrofa; Porcine (Pig)
E90823Po 90kDa热休克蛋白β1(HSP90β1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1) Sus scrofa; Porcine (Pig)
E90867Hu 脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2) Homo sapiens (Human)
E90867Ra 脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2) Rattus norvegicus (Rat)
E90867Mu 脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2) Mus musculus (Mouse)
E90868Hu 内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3) Homo sapiens (Human)
E90868Ra 内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3) Rattus norvegicus (Rat)
E90868Mu 内皮型一氧化氮合酶(NOS3)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3) Mus musculus (Mouse)
E90837Ra 诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2) Rattus norvegicus (Rat)
E90837Mu 诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2) Mus musculus (Mouse)
E90837Rb 诱导型一氧化氮合酶(NOS2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2) Oryctolagus cuniculus (Rabbit)
E90628Ra 一氧化氮合酶运输因子(NOSTRIN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN) Rattus norvegicus (Rat)
E90628Mu 一氧化氮合酶运输因子(NOSTRIN)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN) Mus musculus (Mouse)
E90698Hu 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Homo sapiens (Human)
E90023Mu 趋化因子C-C-基元受体8(CCR8)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Chemokine C-C-Motif Receptor 8 (CCR8) Mus musculus (Mouse)
E90074Hu 免疫球蛋白G1(IgG1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Immunoglobulin G1 (IgG1) Homo sapiens (Human)
E90577Ge 环磷酸鸟苷(cGMP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Cyclic Guanosine Monophosphate (cGMP) General
E90928Hu 肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Protein p53 (TP53) Homo sapiens (Human)
E90928Mu 肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Protein p53 (TP53) Mus musculus (Mouse)
E90928Ra 肿瘤蛋白p53(TP53)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tumor Protein p53 (TP53) Rattus norvegicus (Rat)
E90393Ra P物质(SP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Substance P (SP) Rattus norvegicus (Rat)
E90393Mu P物质(SP)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Substance P (SP) Mus musculus (Mouse)
E90392Hu 速激肽受体1(TACR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tachykinin Receptor 1 (TACR1) Homo sapiens (Human)
E90392Ra 速激肽受体1(TACR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tachykinin Receptor 1 (TACR1) Rattus norvegicus (Rat)
E90392Mu 速激肽受体1(TACR1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Tachykinin Receptor 1 (TACR1) Mus musculus (Mouse)
E90808Ge 5-羟色胺(5-HT)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for 5-Hydroxytryptamine (5-HT) General
E90900Ge γ-氨基丁酸(γABA)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Gamma-Aminobutyric Acid (gABA) General
E90925Hu 多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dopamine Receptor D4 (DRD4) Homo sapiens (Human)
E90925Ra 多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dopamine Receptor D4 (DRD4) Rattus norvegicus (Rat)
E90160Ra α2-纤溶酶抑制因子(α2PI)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Alpha-2-Plasmin Inhibitor (a2PI) Rattus norvegicus (Rat)
E90925Mu 多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dopamine Receptor D4 (DRD4) Mus musculus (Mouse)
E90925Ca 多巴胺受体D4(DRD4)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Dopamine Receptor D4 (DRD4) Canis familiaris; Canine (Dog)
E90588Hu M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Pyruvate Kinase, Muscle (PKM2) Homo sapiens (Human)
E90588Ra M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Pyruvate Kinase, Muscle (PKM2) Rattus norvegicus (Rat)
E90588Mu M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for Pyruvate Kinase, Muscle (PKM2) Mus musculus (Mouse)