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产品名称： 罗氏地高辛标记检测试剂盒KitI
英文名称： DIG-High Prime DNA Labeling ａnd Detection Starter Kit I
产品货号： 11745832910
产品规格： 12LR;24DR
产品产地： ROCHE
优惠价格： 4200
DIG-High Prime DNA Labeling ａnd Detection Starter Kit I
英文名称： DIG-High Prime DNA Labeling ａnd Detection Starter Kit I
产品货号： 11745832910
产品规格： 12LR;24DR
产品产地： ROCHE
优惠价格： 4200
DIG-High Prime DNA Labeling ａnd Detection Starter Kit I
Application
DIG-High Prime labeled probes can be used in Southern-, northern- ａnd dot-blot analysis, as well as colony ａnd plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, ａnd color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, ａnd DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, ａnd reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits. Plasmid, cosmid, ａnd lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.
Product Description
Sensitivity ａnd specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, ａnd 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development （probe concentrations at 20 ng/ml）. A single-copy human gene （tPA） is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
Contents
1. DIG-High Prime, 5x conc., 50 µl
2. DIG-labeled Control DNA, 20 µl, （5 µg/ml）, pBR328 （linearized with Bam HI）
3. DNA Dilution Buffer, 3 x 1 ml
4. Anti-digoxigenin-AP Conjugate, 100 µl
5. NBT/BCIP Stock Solution, conc., 6 x 1 ml
6. Blocking Solution, 10x conc., 4 x 100 ml
DIG Easy Hyb Granules, 4 x 100 ml
