本公司经销Cullin 3，Cullin3抗体，克隆类型为polyclonal，宿主来源是Rabbit，Cullin 3Cullin3抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-4211R
英文名称：Anti-Cullin 3
中文名称：Cullin3抗体
其他名称：CUL 3; Cul-3; CUL3; CUL3_HUMAN; Cullin-3; Cullin3; KIAA0617.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 85kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, hrs, dog, chk, pig, cow, Rb
产品介绍：Core component of multiple cullin-RING-based BCR （BTB-CUL3-RBX1） E3 ubiquitin-protein ligase complexes which mediate the ubiquitination ａnd subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate ａnd the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit ａnd is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 （By similarity）. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR（SPOP） is involved in ubiquitination of BMI1/PCGF4, H2AFY ａnd DAXX, ａnd probably GLI2 or GLI3. BCR（KLHL9-KLHL13） controls the dynamic behavior of AURKB on mitotic chromosomes ａnd thereby coordinates faithful mitotic progression ａnd completion of cytokinesis. Involved in ubiquitination of cyclin E ａnd of cyclin D1 （in vitro） thus involved in regulation of G1/S transition.Function : Core component of multiple cullin-RING-based BCR （BTB-CUL3-RBX1） E3 ubiquitin-protein ligase complexes which mediate the ubiquitination ａnd subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate ａnd the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit ａnd is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 （By similarity）. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR（SPOP） is involved in ubiquitination of BMI1/PCGF4, H2AFY ａnd DAXX, ａnd probably GLI2 or GLI3. BCR（KLHL9-KLHL13） controls the dynamic behavior of AURKB on mitotic chromosomes ａnd thereby coordinates faithful mitotic progression ａnd completion of cytokinesis. BCR（KLHL12） is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem （ES） cells division: BCR（KLHL12） acts by mediating monoubiquitination of SEC31 （SEC31A or SEC31B）. BCR（KLHL3） acts as a regulator of ion transport in the distal nephron; possibly by mediating ubiquitination of SLC12A3/NCC. Involved in ubiquitination of cyclin E ａnd of cyclin D1 （in vitro） thus involved in regulation of G1/S transition.Subunit : Forms neddylation-dependent homodimers. Component of multiple BCR （BTB-CUL3-RBX1） E3 ubiquitin-protein ligase complexes formed of CUL3, RBX1 ａnd a variable BTB domain-containing protein acting as both, adapter to cullin ａnd substrate recognition subunit. The BCR complex may be active as a heterodimeric complex, in which NEDD8, covalently attached to one CUL3 molecule, binds to the C-terminus of a second CUL3 molecule. Interacts with RBX1, RNF7, CYCE ａnd TIP120A/CAND1. Part of the BCR（SPOP） containing SPOP. Part of the probable BCR（KLHL9-KLHL13） complex with BTB domain proteins KLHL9 ａnd KLHL13. Part of the BCR（KBTBD10） complex containing KBTBD10. Component of the BCR（KLHL12） E3 ubiquitin ligase complex, at least composed of CUL3 ａnd KLHL12 ａnd RBX1. Component of the BCR（KLHL3） E3 ubiquitin ligase complex, at least composed of CUL3 ａnd KLHL3 ａnd RBX1 （Probable）. Part of the BCR（ENC1） complex containing ENC1. Part of a complex consisting of BMI1/PCGF4, CUL3 ａnd SPOP. Part of a complex consisting of H2AFY, CUL3 ａnd SPOP. Interacts with KCTD5, KLHL9, KLHL13, GAN, ZBTB16, KLHL21, KLHL3, KLHL15, KLHL20, C16orf44, GMCL1P1, BTBD1. Part of a complex that contains CUL3, RBX1 ａnd GAN. Interacts （via BTB domain） with KLHL17; the interaction regulates surface GRIK2 expression.Subcellular Location : Nucleus. Golgi apparatus.Tissue Specificity : Widely expressed. [PTM] Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome （CSN） complex.DISEASE : Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E （PHA2E） [MIM:614496]. An autosomal dominant disorder characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, ａnd correction of physiologic abnormalities by thiazide diuretics.Similarity : Belongs to the cullin family.