本公司经销phospho-c-Abl（Tyr226），磷酸化非受体酪氨酸激酶c-Abl抗体，克隆类型为polyclonal，宿主来源是Rabbit，phospho-c-Abl（Tyr226）磷酸化非受体酪氨酸激酶c-Abl抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-3041R
英文名称：Anti-phospho-c-Abl（Tyr226）
中文名称：磷酸化非受体酪氨酸激酶c-Abl抗体
其他名称：Alternative names for c Abl （phospho Tyr226） antibody; Abelson Murine Leukemia Viral Oncogene Homolog 1; Abelson murine leukemia viral v abl oncogene homolog 1; Abl 1;ABL;Abl protein; Abl1;Bcr/c abl oncogene protein; JTK 7; JTK7;p150; Proto oncogene tyrosine protein kinase ABL1; Transformation gene oncogene ABL;v abl Abelson murine leukemia viral oncogene homolog 1; v abl;
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 124kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, cow, pig, hrs, Rb, dog
产品介绍：The ABL1 protooncogene encodes a cytoplasmic ａnd nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, ａnd stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, ａnd deletion of the SH3 domain turns ABL1 into an oncogene. The t（9;22） translocation results in the head-to-tail fusion of the BCR （MIM:151410） ａnd ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq].Function : Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth ａnd survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility ａnd adhesion, receptor endocytosis, autophagy, DNA damage response ａnd apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 （involved in branch formation）; ANXA1 （involved in membrane anchoring）; DBN1, DBNL, CTTN, RAPH1 ａnd ENAH （involved in signaling）; or MAPT ａnd PXN （microtubule-binding proteins）. Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation ａnd cell migration. Involved in the regulation of cell adhesion ａnd motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases ａnd more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis ａnd modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin （CAV1） ａnd RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation ａnd actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking ａnd function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress ａnd thereby mediates mitochondrial dysfunction ａnd cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity ａnd is involved in DNA-damage response ａnd apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity ａnd cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir （translocated intimin receptor） of pathogenic E.coli ａnd possibly Citrobacter, CagA （cytotoxin-associated gene A） of H.pylori, or AnkA （ankyrin repeat-containing protein A） of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement ａnd host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.Subunit : Interacts with SORBS1 following insulin stimulation. Found in a trimolecular complex containing CDK5 ａnd CABLES1. Interacts with CABLES1 ａnd PSTPIP1. Interacts with ZDHHC16, ITGB1 ａnd HCK （By similarity）. Interacts with INPPL1/SHIP2. Interacts with the 14-3-3 proteins, YWHAB, YWHAE, YWHAG, YWHAH, SFN AND YWHAZ; the interaction with 14-3-3 proteins requires phosphorylation on Thr-735 and, sequesters ABL1 into the cytoplasm. Interacts with ABI1, ABI2, BCR, CRK, FGR, FYN, HCK, LYN, PSMA7 RAD9A, RAD51, RAD52, TP73 ａnd WASF3. A complex made of ABL1, CTTN ａnd MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate （S1P）-mediated endothelial cell （EC） barrier enhancement.Subcellular Location : Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Note=Shuttles between the nucleus ａnd cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress. Isoform IB: Nucleus membrane; Lipid-anchor. Note=The myristoylated c-ABL protein is reported to be nuclear.Tissue Specificity : Widely expressed.Post-translational modifications : Acetylated at Lys-711 by EP300 which promotes the cytoplasmic translocation.Phosphorylation at Tyr-70 by members of the SRC family of kinases disrupts SH3 domain-based autoinhibitory interactions ａnd intermolecular associations, such as that with ABI1, ａnd also enhances kinase activity. Phosphorylation at Tyr-226 ａnd Tyr-393 correlate with increased activity. DNA damage-induced activation of ABL1 requires the function of ATM ａnd Ser-446 phosphorylation. Phosphorylation at Ser-569 has been attributed to a CDC2-associated kinase ａnd is coupled to cell division. Phosphorylation at Ser-618 ａnd Ser-619 by PAK2 increases binding to CRK ａnd reduces binding to ABI1. Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Phosphorylated by PRKDC.Polyubiquitinated. Polyubiquitination of ABL1 leads to degradation.Isoform IB is myristoylated on Gly-2.DISEASE : Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t（9;22）（q34;q11） with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia （AML） ａnd acute lymphoblastic leukemia （ALL）.Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.非受体酪氨酸激酶c-Abl广泛表达于各组织细胞中,c-Abl是非受体酪氨酸激酶Src家族的一个成员。在生理状态下,它可以定位于多个亚细胞结构（如细胞核、细胞质、线粒体等）中并呈现不同功能。经研究认为,细胞核内的c-Abl在细胞凋亡调控以及DNA损伤修复过程中起重要作用,而胞质中的c-Abl则与细胞黏附、细胞分化及氧化应激有关联，该蛋白主要用于细胞凋亡的研究