本公司经销phospho-CDKN1B/P27kip1 （Ser10），磷酸化P27抗体/周期素依赖激酶抑制剂，克隆类型为polyclonal，宿主来源是Rabbit，phospho-CDKN1B/P27kip1 （Ser10）磷酸化P27抗体/周期素依赖激酶抑制剂可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-5227R
英文名称：Anti-phospho-CDKN1B/P27kip1 （Ser10）
中文名称：磷酸化P27抗体/周期素依赖激酶抑制剂
其他名称：cyclin-dependent kinase inhibitor 1B; p27, kip1; Cdkn1b; Cyclin- dependent kinase inhibitor p27; p27Kip1.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 22kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo
产品介绍：Cell cycle progression is regulated by cyclins ａnd their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, ａnd has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4.p27 Kip1 is regulated by phosphorylation on serine 10 （S10） ａnd threonine 187 （T187）. Phosphorylation by CDK2 on T187 results in ubiquitylation ａnd degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm. Function : Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- ａnd cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes ａnd is involved in the assembly, stability, ａnd modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.Subunit : Forms a ternary compex with CCNE1/CDK2/CDKN1B.Subcellular Location : Nucleus. Cytoplasm. Endosome. Note=Nuclear ａnd cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm ａnd promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm ａnd cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 ａnd Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation.Tissue Specificity : Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver ａnd kidney.Post-translational modifications : Phosphorylated; phosphorylation occurs on serine, threonine ａnd tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G（0）-G（1） phase ａnd leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC ａnd in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination ａnd proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 ａnd Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.Ubiquitinated; in the cytoplasm by the KPC complex （composed of RNF123/KPC1 ａnd UBAC1/KPC2） and, in the nucleus, by SCF（SKP2）. The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF（SKP2）-like complex; leads to its degradation.DISEASE : Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 （MEN4） [MIM:610755]. Multiple endocrine neoplasia （MEN） syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 ａnd MEN2.Similarity : Belongs to the CDI family.P27蛋白是一种新发现的周期素依赖激酶抑制剂，属于细胞周期的负性调控因子。P27基因及其产物的异常表达可能与某些肿瘤的发生、发展有着密切的关系。P27蛋白对细胞周期的调控及在肿瘤中发挥着很重要的作用。