本公司经销Phospho-Caspase-9 （Ser196），磷酸化半胱氨酸蛋白酶9抗体，克隆类型为polyclonal，宿主来源是Rabbit，Phospho-Caspase-9 （Ser196）磷酸化半胱氨酸蛋白酶9抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-5241R
英文名称：Anti-Phospho-Caspase-9 （Ser196）
中文名称：磷酸化半胱氨酸蛋白酶9抗体
其他名称：APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; CASP 9; CASP9; Caspase 9 Dominant Negative; Caspase 9 precursor; Caspase 9c; Caspase9; EC 3.4.22.; ICE LAP6; ICE like apoptotic protease 6; MCH6 antibody RNCASP9.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 52kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat
产品介绍：Caspase 9 （also known as ICE like apoptotic protease 6 （ICE LAP6）, apoptotic protease Mch6, ａnd apoptotic protease activating factor 3 （Apaf3）） is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer ａnd has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution ａnd cleaves/activates Caspase 3 ａnd Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, ａnd Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, ａnd following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 ａnd Caspase 10 into large p37 ａnd small p10 subunits. Caspase 9 intereacts with BIRC7 ａnd has been shown to cleave PARP ａnd vimentin.Function : Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves ａnd activates caspase-3. Proteolytically cleaves poly（ADP-ribose） polymerase （PARP）.Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.Subunit : Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa （p35） ａnd a 10 kDa （p10） subunit. Caspase-9 ａnd APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C ａnd ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 ａnd BIRC7. Interacts （inactive form） with EFHD2. Interacts with HAX1.Tissue Specificity : Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, ａnd pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.Post-translational modifications : Cleavages at Asp-315 by granzyme B ａnd at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 ａnd -10 can also be involved in these processing events.Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing ａnd subsequent caspase-3 activation.Similarity : Belongs to the peptidase C14A family.Contains 1 CARD domain.Caspase-9半胱氨酸蛋白酶家族成员，又称ICE-Lap6（ICE Like apoptotease 6）参与细胞凋亡过程和细胞因子的加工过程，在许多胚胎和成人组织中都有分布。此抗体主要用于肿瘤凋亡的研究。