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CD10/CALLA/NeprilysinCD10抗体
点击次数:277发布时间:2012/12/5 11:25:01
更新日期:2024/9/5 14:43:01
所 在 地:中国大陆
产品型号:BY-0527R
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详细内容
货号:BY-0527R
英文名称:Anti-CD10/CALLA/Neprilysin
中文名称:CD10抗体
其他名称:Atriopeptidase; CALLA; CD 10; CD10; CD10; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase.
抗体来源:Rabbit
克隆类型:polyclonal
蛋白分子量:predicted molecular weight: 85-95kDa
纯化方法:affinity purified by Protein A
交叉反应:hu, mo, rat
产品介绍:CD10 is a 100kD glycoprotein, also designated Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral etalloendopeptidase activity which inactivates a variety of biologically active peptides.CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.Subcellular Location : Cell membrane; Single-pass type II membrane protein.Similarity : Belongs to the peptidase M13 family.CD10即共同型急性淋巴细胞白血病抗原,是II型跨膜糖蛋白.CD10表达于早期B淋巴细胞,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的肿瘤细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型.CD10是一种94kD的锌依赖性细胞膜金属结合蛋白,参与细胞神经肽和激素肽的后分泌过程。它广泛地分布于肾、肝、小肠、胎盘、脉络膜丛、脑腺、腺皮质和白细胞,CD10是生发中心细胞及所致淋巴瘤的标志物。产品图片Tissue/cell: mouse small intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD10 Polyclonal Antibody, Unconjugated(bs-0527R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei