本公司经销Dnmt3a，DNA甲基转移酶-3α抗体，克隆类型为polyclonal，宿主来源是Rabbit，Dnmt3aDNA甲基转移酶-3α抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0497R
英文名称：Anti-Dnmt3a
中文名称：DNA甲基转移酶-3α抗体
其他名称：名DNA （cytosine 5 ） methyltransferase 3 alpha; DNA （cytosine 5） methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 100kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, cow
产品介绍：The tumor organization has the DNA methylation disorder, including ａnd damps cancer gene high methylation DNA with the cell multiplication cycle close related cancer gene low methylation the methyl transferase （Dnmt） participation methylation the formation （is mainly Dnmt3a ａnd Dnmt3b） ａnd the maintenance （is mainly Dnmt1） （isoform CRA_b） （DNA methyltransferase MmuIIIA） （DNA MTase MmuIIIA）Function : Required for genome-wide de novo methylation ａnd is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner ａnd also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores ａnd is inhibited by histone H1. Plays a role in paternal ａnd maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.Subunit : Heterotetramer composed of 1 DNMT3A homodimer ａnd 2 DNMT3L subunits （DNMT3L-DNMT3A-DNMT3A-DNMT3L）. Interacts with UBC9, PIAS1 ａnd PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with DNMT1 ａnd DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at ＇Lys-4＇ （H3K4）.Subcellular Location : Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.Tissue Specificity : Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, ａnd at lower levels in placenta, brain, liver, colon, spleen, small intestine ａnd lung.Post-translational modifications : Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases （HDAC1 ａnd HDAC2） ａnd repress transcription.Similarity : Belongs to the C5-methyltransferase family. Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain.肿瘤组织存在DNA甲基化紊乱，包括与细胞增殖周期密切相关的癌基因低甲基化和抑癌基因高甲基化DNA甲基转移酶（Dnmt）参与甲基化的形成（主要是Dnmt3a和Dnmt3b）和维持（主要是Dnmt1）产品图片Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated（bs-0497R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingTissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated（bs-0497R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） staining