本公司经销Dnmt3 Beta，DNA甲基转移酶-3β抗体，克隆类型为polyclonal，宿主来源是Rabbit，Dnmt3 BetaDNA甲基转移酶-3β抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0301R
英文名称：Anti-Dnmt3 Beta
中文名称：DNA甲基转移酶-3β抗体
其他名称：名Cytosine 5methyltransferase 3B; DNA （cytosine 5） methyltransferase 3 beta; DNA; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3b; EC 2.1.1.37; ICF; M.HsaIIIB; MGC124407; RP23-89H14.3.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 94kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat
产品介绍：Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting ａnd is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 ａnd Dnmt3. Dnmt1 is constitutively expressed in proliferating cells ａnd inactivation of this gene causes global demethylation of genomic DNA ａnd embryonic lethality. Dnmt2 is expressed at low levels in adult tissues ａnd its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a ａnd Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells ａnd is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF （immunodeficiency, centromeric instability, ａnd facial anomalies） syndrome.Function : Required for genome-wide de novo methylation ａnd is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 ａnd independently of DNA methylation. Seems to be involved in gene silencing （By similarity）. In association with DNMT1 ａnd via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 ａnd H3K9. Isoforms 4 ａnd 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.Subunit : Interacts with BAZ2A/TIP5, SUV39H1 ａnd CBX4. Interacts with DNMT1 ａnd DNMT3A, SETDB1, UBL1, UBE2I9 ａnd ZHX1. Interacts with the PRC2/EED-EZH2 complex.Subcellular Location : Nucleus.Tissue Specificity : Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, ａnd at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, ａnd skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle ａnd PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung ａnd prostate ａnd 5 is detectable only in testis ａnd at very low level in brain ａnd prostate.Post-translational modifications : Sumoylated.DISEASE : Defects in DNMT3B are a cause of immunodeficiency-centromeric instability-facial anomalies syndrome type 1 （ICF1） [MIM:242860]. A rare disorder characterized by a variable immunodeficiency, facial anomalies, ａnd branching of chromosomes 1, 9, ａnd 16. Other variable symptoms include growth retardation, failure to thrive, ａnd psychomotor retardation. Laboratory studies show limited hypomethylation of DNA in a small fraction of the genome in some, but not all, patients.Similarity : Belongs to the C5-methyltransferase family.Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain.肿瘤组织存在DNA甲基化紊乱，包括与细胞增殖周期密切相关的癌基因低甲基化和抑癌基因高甲基化DNA甲基转移酶（Dnmt）参与甲基化的形成（主要是Dnmt3a和Dnmt3b）和维持（主要是Dnmt1）。产品图片Tissue/cell: rat spinal cord tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-Dnmt-3-beta Polyclonal Antibody, Unconjugated（bs-0301R） 1:600, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingOverlay histogram showing Mouse spleen cells stained with bs-0301R-PE （red line）. The cells were fixed with 1% paraformaldehyde （10 min）. The cellswere then incubated with the antibody（bs-0301R-PE, 2ug/1x106cells） for 30 min at 22-25°C. Isotypecontrol antibody （black line） was rabbit IgG （2ug/1x106cells）used under the same conditions.Acquisition of events were used for analysis.