本公司经销DNAPK/PRKDC，DNA依赖蛋白激酶催化亚基抗体，克隆类型为polyclonal，宿主来源是Rabbit，DNAPK/PRKDCDNA依赖蛋白激酶催化亚基抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-1359R
英文名称：Anti-DNAPK/PRKDC
中文名称：DNA依赖蛋白激酶催化亚基抗体
其他名称：名DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; Variant; DNAPK; DNAPK catalytic subunit; p350; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 454kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, cow, hrs, chk, dog, Rb
产品介绍：Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining （NHEJ） required for double-strand break （DSB） repair ａnd V（D）J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V（D）J recombination by activation of the hairpin endonuclease artemis （DCLRE1C）. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect ａnd align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability ａnd the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ＇Ser-139＇ of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC ａnd RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate TP53/p53 in the presence of supercoiled DNA is dependent on C1D.Function : Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining （NHEJ） required for double-strand break （DSB） repair ａnd V（D）J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V（D）J recombination by activation of the hairpin endonuclease artemis DCLRE1C）. The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect ａnd align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability ａnd the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates ＇Ser-139＇ of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC ａnd RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.Subunit : DNA-PK is a heterotrimer of PRKDC ａnd the Ku p70-p86 （XRCC6-XRCC5） dimer. Formation of this complex may be promoted by interaction with ILF3. Associates with the DNA-bound Ku heterodimer, but it can also bind to ａnd be activated by free DNA. Interacts with DNA-PKcs-interacting protein （KIP） with the region upstream the kinase domain. PRKDC alone also interacts with ａnd phosphorylates DCLRE1C, thereby activating the latent endonuclease activity of this protein. Interacts with C1D. Interacts with TTI1 ａnd TELO2.Subcellular Location : Nucleus.Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 ａnd Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site （inducible with ionizing radiation, IR）. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing ａnd DNA repair.S-nitrosylated by GAPDH.Similarity : Belongs to the PI3/PI4-kinase family.Contains 1 FAT domain. Contains 1 FATC domain. Contains 2 HEAT repeats. Contains 1 PI3K/PI4K domain. Contains 3 TPR repeats.DNA依赖的蛋白激酶（DNA-PK）是一种DNA活化的核丝氨酸苏氨酸蛋白激酶. 参与体内DNA双链断裂缺口的修复, 在肿瘤的预后、发生、发展、诊断和治疗等研究中引起科研人员越来越多的重视。