本公司经销ER-alpha/beta，雌激素受体α/β抗体，克隆类型为polyclonal，宿主来源是Rabbit，ER-alpha/beta雌激素受体α/β抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0174R
英文名称：Anti-ER-alpha/beta
中文名称：雌激素受体α/β抗体
其他名称：名Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER Beta; ER[a]; ER[b]; ERA; ERalpha; Erb; Erb2; ERbeta; ESR 1; ESR; ESR BETA; ESR1; ESR2; ESRA; ESRB; Estr; Estra; Estradiol Receptor alpha; Estradiol Receptor; Estradiol Receptor beta; ESTRB; Estrogen Receptor; Estrogen receptor 1 （alpha）; Estrogen receptor 1; Estrogen receptor 2 （ER beta）; Estrogen Receptor 2; Estrogen receptor 2 ER beta; Estrogen receptor alpha; Estrogen receptor beta 4; Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A 1; NR3A1; NR3A2; Nuclear receptor subfamily 3 group A member 1; Nuclear receptor subfamily 3 group A member 2; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 67kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, pig
产品介绍：The estrogen receptor （ER） is a 66 kDa protein that mediates the actions of estrogens in estrogen responsive tissues. It is a member of a large superfamily of nuclear hormone receptors that function as ligand activated transcription factors. The ER gene consists of more than 140 kb of genomic DNA divided into 8 exons. These translate into a protein with six functionally discrete domains, labeled A through F. A second form of the estrogen receptor, ER beta has recently been described. The ER is an important regulator of growth ａnd differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti estrogen （e.g. tamoxifen） therapy.类固醇受体（Steroid Receptors）产品图片Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-ER-alpha/beta Polyclonal Antibody, Unconjugated（bs-0174R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingProtein: 1.rat brain lysates, 30ug; 2.rat colon lysates, 30ugPrimary: Anti-ER-alpha/beta （bs-0174R） at 1:200Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bse-0295G） at 1: 3000Predicted band size : 58kD,66kDObserved band size : 58kDER-alpha: 66kD; ER-beta: 58kD; Antigen: bs-0174P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti- Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.