本公司经销AGER，晚期糖基化终末产物特异性受体抗体，克隆类型为polyclonal，宿主来源是Rabbit，AGER晚期糖基化终末产物特异性受体抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0177R
英文名称：Anti-AGER
中文名称：晚期糖基化终末产物特异性受体抗体
其他名称：Advanced glycosylation end product specific receptor; Advanced glycosylation end product-specific receptor; AGER; EC 2.7.11.22; LE 9211 A antigen;LE-9211-A antigen; MGC22357; MOK; RAGE 1; RAGE1; MOK protein kinase; Receptor for advanced glycation endproducts;Renal tumor antigen 1; Renal tumor antigen; Renal cell carcinoma antigen （MOK protein kinase）;Renal tumor antigen 1.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 42kDa
纯化方法：affinity purified by Protein A
交叉反应：rat
产品介绍：Advanced glycosylation end product-specific receptor （AGER; RAGE） is a member of the immunoglobulin superfamily of cell surface molecules that binds molecules that have been irreversibly modified by non-enzymatic glycation ａnd oxidation, ａnd are know as advanced glycation end products （AGEs）. It is expressed by endothelium, mononuclear phagocytes, neurons ａnd smooth muscle cells. Whereas RAGE is present at high levels during development, especially in the central nervous system, its levels decline during maturity.The increased expression of RAGE is associated with several pathological states, such as diabetic vasculopathy, neuropathy, retinopathy ａnd other disorders, including Alzheimer＇s disease ａnd immune/inflammatory reactions of the vessel walls. In diabetic tissues, the production of RAGE is due to the overproduction of AGEs that eventually overwhelm the protective properties of RAGE. This results in oxidative stress ａnd endothelial cell dysfunction that leads to vascular disease in diabetics. In the brain, RAGE also binds amyloid beta （Ab）. Because Ab is overproduced in neurons ａnd vessels in the brains of Alzheimer disease, this leads to the hyperstimulation of RAGE. The RAGE-Ab interaction is thought to result in oxidative stress leading to neuronal degeneration. Function : Mediates interactions of advanced glycosylation end products （AGE）. These are nzymatically glycosylated proteins which accumulate in vascular tissue in aging ａnd at an accelerated rate in diabetes. Acts as a mediator of both acute ａnd chronic vascular inflammation in conditions such as atherosclerosis ａnd in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, ａnd endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, ａnd lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide （ABPP） across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase （MAPK）, has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 ａnd p53/TP53 signaling.Subunit : Interacts with S100B, S100A1 ａnd APP （By similarity）. Interacts with S100A12.Subcellular Location : Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.Tissue Specificity : Endothelial cells ａnd cardiomyocytes.Similarity : Contains 2 Ig-like C2-type （immunoglobulin-like） domains.Contains 1 Ig-like V-type （immunoglobulin-like） domain.晚期糖基化终末产物受体（AGER）与其配体AGEs形成的AGEs-AGER 系统在糖尿病血管病变的发生、发展过程中起着重要作用. 年龄及晚期糖基化终末产物（AGEs）等多种因素均能调节AGER基因的表达. 糖尿病患者体内晚期糖基化终末产物受体（AGER）的高表达加速了病人血管病变的发展过程,并增加了病变的复杂性.阻断AGER通路可缓解糖尿病血管的病变过程。 因此,AGER可以作为治疗糖尿病血管病变的药物靶点,并为临床治疗糖尿病血管病变提供了新的思路.产品图片Tissue/cell: rat sciatic nerve; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-AGER/RAGE Polyclonal Antibody, Unconjugated（bs-0177R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingProtein: line1, rat kidney lysates, 30ug; line2, rat brain lysates, 30ug; Primary: Anti-AGER （bs-0177R） at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bse-0295G） at 1: 3000; ECL excitated the fluorescence; Predicted band size : 44kDObserved band size : 80kDMay be formed of homo-dimerAntigen: bs-0177P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti- Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.