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Anti-AGPAT4溶血磷脂酸酰基转移酶D抗体
点击次数:204发布时间:2012/12/26 4:09:44
更新日期:2024/9/5 14:43:27
所 在 地:其它
产品型号:BY-5033R
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详细内容
货号:BY-5033R
英文名称:Anti-AGPAT4
中文名称:溶血磷脂酸酰基转移酶D抗体
其他名称:1 acyl sn glycerol 3 phosphate acyltransferase delta; 1 acylglycerol 3 phosphate O acyltransferase 4; 1 AGP acyltransferase 4; LPAAT delta; Lysophosphatidic acid acyltransferase delta.
抗体来源:Rabbit
克隆类型:polyclonal
蛋白分子量:predicted molecular weight: 41kDa
纯化方法:affinity purified by Protein A
交叉反应:hu, mo, rat, hrs, dog, pig, Rb, cow
产品介绍:Agpat4 converts lysophosphatidic acid into phosphatidic acid by incorporating acyl moiety at the 2 position. The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.Subcellular Location : Membrane; Multi-pass membrane protein.Similarity : Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.产品图片Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AGPAT4 Polyclonal Antibody, Unconjugated(bs-5033R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: mouse cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AGPAT4 Polyclonal Antibody, Unconjugated(bs-5033R) 1:200,overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingProtein: line1, rat brain lysates, 30ug; line2, rat heart lysates, 30ug; Primary: Anti-AGPAT4 (bs-5033R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 41kDObserved band size : 41kD