本公司经销BIRC5/Survivin variant，细胞凋亡抑制因子5抗体，克隆类型为polyclonal，宿主来源是Rabbit，BIRC5/Survivin variant细胞凋亡抑制因子5抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0726R
英文名称：Anti-BIRC5/Survivin variant
中文名称：细胞凋亡抑制因子5抗体
其他名称：API4; Apoptosis Inhibitor 4; Survivin 2B; Survivin2B; Survivin-2B; Apoptosis inhibitor survivin; Apoptosis inhibitor4; Baculoviral IAP repeat containing 5; Baculoviral IAP repeat containing protein 5; BIRC 5; BIRC5; EPR 1; IAP4; SVV; TIAP.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 18.5kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo, cow, dog, pig
产品介绍：Shepherdin （Survivin variant; baculoviral IAP repeat-containing protein 5 isoform 3; BIRC5）. Baculoviral inhibition of apoptosis protein repeat domain; Found in inhibitors of apoptosis proteins （IAPs） ａnd other proteins. In higher eukaryotes, BIR domains inhibit apoptosis by acting as direct inhibitors of the caspase family of protease enzymes. In yeast, BIR domains are involved in regulating cytokinesis. This novel fold is stabilized by zinc tetrahedrally coordinated by one histidine ａnd three cysteine residues ａnd resembles a classical zinc finger.Survivin is an anti-apoptotic protein, expressed in the G2/M cell cycle phase. At the onset of mitosis, survivin associates with microtubules of the mitotic spindle in a specific ａnd saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin＇s anti-apoptotic function ａnd increased caspase 3 activity.Survivin is also abundantly expressed in brain tissues （astrocytes ａnd some neurons） of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN （mature neuronal marker） ａnd PCNA （a cell cycle protein. Survivin might affect regulation of neural cell proliferative responses after brain injury. Survivin is also an inhibitor of apoptosis （IAP）, or programmed cell death, that is selectively overexpressed in common human cancers.Function : Multitasking protein that has dual roles in promoting cell proliferation ａnd preventing apoptosis. Component of a chromosome passage protein complex （CPC） which is essential for chromosome alignment ａnd segregation during mitosis ａnd cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis ａnd participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 ａnd CASP7. Isoform 2 ａnd isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.Subunit : Monomer or homodimer. Exists as a homodimer in the apo state ａnd as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently. Component of the chromosomal passenger complex （CPC） composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB ａnd AURKC. Interacts with JTB. Interacts with CDCA8 ａnd INCENP; interaction is direct. Interacts with EVI5. Interacts with GTP-bound RAN in both the S ａnd M phases of the cell cycle. Interacts with USP9X. Interacts with tubulin. Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric ａnd monomeric form can interact with DIABLO/SMAC. Interacts with BIRC6/bruce.Subcellular Location : Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms ａnd inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase ａnd at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization ａnd thereby inhibiting XPO1/CRM1-mediated nuclear export.Tissue Specificity : Expressed only in fetal kidney ａnd liver, ａnd to lesser extent, lung ａnd brain. Abundantly expressed in adenocarcinoma （lung, pancreas, colon, breast, ａnd prostate） ａnd in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.Post-translational modifications : Ubiquitination is required for centrosomal targeting.In vitro phosphorylation at Thr-117 by AURKB prevents interaction with INCENP ａnd localization to mitotic chromosomes. Phosphorylation at Thr-48 by CK2 is critical for its mitotic ａnd anti-apoptotic activities. Acetylation at Lys-129 by CBP results in its homodimerization, while deacetylation promotes the formation of monomers which heterodimerize with XPO1/CRM1 which facilitates its nuclear export. The acetylated form represses STAT3 transactivation. The dynamic equilibrium between its acetylation ａnd deacetylation at Lys-129 determines its interaction with XPO1/CRM1, its subsequent subcellular localization, ａnd its ability to inhibit STAT3 transactivation.Similarity : Belongs to the IAP family.Contains 1 BIR repeat.为Survivin家族成员，又称：细胞凋亡抑制因子4，主要用于肿瘤方面的研究。产品图片Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-BIRC5 Polyclonal Antibody, Unconjugated（bs-0726R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） staining