本公司经销phospho-B-Raf （Ser446），磷酸化B-Raf抗体，克隆类型为polyclonal，宿主来源是Rabbit，phospho-B-Raf （Ser446）磷酸化B-Raf抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-5232R
英文名称：Anti-phospho-B-Raf （Ser446）
中文名称：磷酸化B-Raf抗体
其他名称：94 kDa B raf protein; B raf 1; B Raf proto oncogene serine threonine protein kinase; BRAF 1; Braf; BRAF1; cRmil; MGC126806; MGC138284; Murine sarcoma viral （v-raf） oncogene homolog B1; Murine sarcoma viral v raf oncogene homolog B1; p94; RAFB 1; RAFB1; v raf murine sarcoma viral oncogene homolog B1; FLJ95109.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 94kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, chk, hrs, pig, dog, cow, Rb
产品介绍：The Raf kinases are important intermediates in signal transduction. Raf protein family members, including A Raf ａnd B Raf, have intrinsic serine/threonine kinase activity. Interaction between Ras proteins ａnd Raf proteins results in Raf-mediated phosphorylation ａnd activation of MEK （also known as MAP kinase kinase）. Defects in BRAF are involved in a wide range of cancers. B-Raf is a serine/threonine protein kinase that acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. 1 BRAF is important for the regulation of cell proliferation ａnd determination of cell fate during embryogenesis. BRAF acts downstream of Ras ａnd upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, ａnd differentiation in response to growth factors, cytokines, ａnd hormones.Function : Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.Subunit : Monomer. Homodimer. Heterodimerizes with RAF1, ａnd the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated ａnd enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS1, MAP2K1, MAPK3 ａnd RGS14. Interacts with RIT1. Interacts （via N-terminus） with RGS14 （via RBD domains）; the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 （By similarity）. Interacts with DGKH.Subcellular Location : Nucleus. Cytoplasm. Cell membrane.Tissue Specificity : Brain ａnd testis.Post-translational modifications : Phosphorylation at Ser-365 by SGK1 inhibits its activity.Methylation at Arg-671 decreases stability ａnd kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation.DISEASE : Note=Defects in BRAF are found in a wide range of cancers. Defects in BRAF may be a cause of colorectal cancer （CRC） [MIM:114500]. Defects in BRAF are involved in lung cancer （LNCR） [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer （NSCLC） that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, ａnd large cell lung cancer. NSCLC is often diagnosed at an advanced stage ａnd has a poor prognosis. Defects in BRAF are involved in non-Hodgkin lymphoma （NHL） [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body＇s immune system. NHLs can occur at any age ａnd are often marked by enlarged lymph nodes, fever ａnd weight loss. Defects in BRAF are a cause of cardiofaciocutaneous syndrome （CFC syndrome） [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects ａnd mental retardation. Heart defects include pulmonic stenosis, atrial septal defects ａnd hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions ａnd a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, ａnd posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.Defects in BRAF are the cause of Noonan syndrome type 7 （NS7） [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant ａnd low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay ａnd variable intellectual deficits.Defects in BRAF are the cause of LEOPARD syndrome type 3 （LEOPARD3） [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, ａnd sensorineural deafness.Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity ａnd inducing cell transformation.Similarity : Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD （Ras-binding） domain.Database links : UniProtKB/Swiss-Prot: P15056.4BRAF蛋白是有丝分裂原活化的蛋白激酶/细胞外信号调节激酶途径蛋白。正常的B-Raf蛋白的功能是传递来自细胞膜的信号,在Ras-Raf-MEK-ERK信号转导调节途径中有着重要的作用。BRAF是一种在癌细胞的生长和存活中起到关键作用的蛋白质,并且在大多数恶性黑素瘤患者和少数结肠癌、乳腺癌和肺癌患者中发生突变，目前BRAF也用于恶性黑素瘤和肿瘤的研究。