本公司经销Caspase-9，白介素1-β转化酶样凋亡蛋白酶6抗体，克隆类型为polyclonal，宿主来源是Rabbit，Caspase-9白介素1-β转化酶样凋亡蛋白酶6抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0050R
英文名称：Anti-Caspase-9
中文名称：白介素1-β转化酶样凋亡蛋白酶6抗体
其他名称：Caspase-9 subunit p35; Apaf-3; APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; CASP 9; CASP9; Caspase 9 Dominant Negative; Caspase 9c; Caspase9; ICE LAP6; ICE like apoptotic protease 6; MCH6; RNCASP9.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 35/50kDa
纯化方法：affinity purified by Protein A
交叉反应：hu
产品介绍：Caspase 9 （also known as ICE like apoptotic protease 6 （ICE LAP6）, apoptotic protease Mch6, ａnd apoptotic protease activating factor 3 （Apaf3）） is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer ａnd has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution ａnd cleaves/activates Caspase 3 ａnd Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, ａnd Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, ａnd following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 ａnd Caspase 10 into large p37 ａnd small p10 subunits. Caspase 9 intereacts with BIRC7 ａnd has been shown to cleave PARP ａnd vimentin.Function : Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves ａnd activates caspase-3. Proteolytically cleaves poly（ADP-ribose） polymerase （PARP）.Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.Subunit : Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa （p35） ａnd a 10 kDa （p10） subunit. Caspase-9 ａnd APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C ａnd ATP. Interacts （inactive form） with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce ａnd BIRC7/livin.Tissue Specificity : Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, ａnd pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.Post-translational modifications : Cleavages at Asp-315 by granzyme B ａnd at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 ａnd -10 can also be involved in these processing events.Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing ａnd subsequent caspase-3 activation.Similarity : Belongs to the peptidase C14A family.Contains 1 CARD domain.Caspase-9半胱氨酸蛋白酶家族成员，又称ICE-Lap6（ICE Like apoptotease 6）参与细胞凋亡过程和细胞因子的加工过程，在许多胚胎和成人组织中都有分布。此抗体主要用于肿瘤研究产品图片Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-Caspase-9 Polyclonal Antibody, Unconjugated（bs-0050R） 1:300, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingAntigen: bs-0050P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti- Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.