本公司经销Cyclin E，周期素E抗体，克隆类型为polyclonal，宿主来源是Rabbit，Cyclin E周期素E抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0573R
英文名称：Anti-Cyclin E
中文名称：周期素E抗体
其他名称：CCNE 1; CCNE; CCNE1; Cyclin E1; Cyclin Es; Cyclin Et; CyclinE; G1/S specific cyclin E.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 45kDa
纯化方法：affinity purified by Protein A
交叉反应：rat, mo
产品介绍：The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression ａnd degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with ａnd functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary ａnd is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, ａnd thus may contribute to tumorigenesis. This protein was found to associate with, ａnd be involved in, the phosphorylation of NPAT protein （nuclear protein mapped to the ATM locus）, which participates in cell-cycle regulated histone gene expression ａnd plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. Transcript Variant: This variant （1） contains a different 5＇ end region, which includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a 15 aa longer N-terminus, as compared to isoform 2. Subunit : Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Found in a complex with CDK2, CABLES1 ａnd CCNA1 （By similarity）. Part of a complex consisting of UHRF2, CDK2 ａnd CCNE1. Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 ａnd appears to occur independently of CCNE1 phosphorylation.Subcellular Location : Nucleus.Tissue Specificity : Highly expressed in testis ａnd placenta. Low levels in bronchial epithelial cells.Similarity : Belongs to the cyclin family. Cyclin E subfamily. 细胞周期素E是调控细胞G-1→S期转变的关键因素。由于在多种肿瘤中的不适当表达,细胞周期素E现在已明确为原癌基因。产品图片Protein: line1, rat brain lysates, 30ug; line2, rat lung lysates, 30ug; Primary: Anti-Cyclin E （bs-0573R） at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bse-0295G） at 1: 3000; ECL excitated the fluorescence; Predicted band size : 45kDObserved band size : 45kDWe are unsure as to the identity of these extra bandsMay be formed of homo-dimerAntigen: bs-0573P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.