本公司经销CYP450，细胞色素P450单氧化酶抗体，克隆类型为polyclonal，宿主来源是Rabbit，CYP450细胞色素P450单氧化酶抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0461R
英文名称：Anti-CYP450
中文名称：细胞色素P450单氧化酶抗体
其他名称：Cytochrome P450 monooxygenase; CPR; CYPOR; DKFZp686G04235; FLJ26468; NADPH Cytochrome P450 Reductase; P450 Cytochrome Oxidoreductase; P450R; POR.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 55kDa
纯化方法：affinity purified by Protein A
交叉反应：rat, mo
产品介绍：Cytochrome P450s are haem-thiolate proteins involved in the oxidative degradation of various compounds. They are particularly well known for their role in the degradation of environmental toxins ａnd mutagens. They can be divided into 4 classes, according to the method by which electrons from NAD（P）H are delivered to the catalytic site. Sequence conservation is relatively low within the family - there are only 3 absolutely conserved residues - but their general topography ａnd structural fold are highly conserved. The conserved core is composed of a coil termed the ＇meander＇, a four-helix bundle, helices J ａnd K, ａnd two sets of beta-sheets. These constitute the haem-binding loop （with an absolutely conserved cysteine that serves as the 5th ligand for the haem iron）, the proton-transfer groove ａnd the absolutely conserved EXXR motif in helix K. While prokaryotic P450s are soluble proteins, most eukaryotic P450s are associated with microsomal membranes. their general enzymatic function is to catalyse regiospecific ａnd stereospecific oxidation of non-activated hydrocarbons at physiological temperatures.CYP单氧酶;细胞色素P450单氧化酶（cytochrome P450 monooxygenase，CYP450） 它参与内源性物质和包括药物、环境化合物在内的外源性物质的代谢.产品图片Tissue/cell: Mouse tumor tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-CYP450 Polyclonal Antibody, Unconjugated（bs-0461R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingTissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-CYP450 Polyclonal Antibody, Unconjugated（bs-0461R） 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated（bs-0295G-PE）used at 1:200 dilution for 40 minutes at 37°C.