本公司经销CD34，CD34抗体，克隆类型为polyclonal，宿主来源是Rabbit，CD34CD34抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-2038R
英文名称：Anti-CD34
中文名称：CD34抗体
其他名称：CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 36kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo
产品介绍：Hematopoietic Stem Cell Marker Endothelial Markers Tumor biomarkers The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, ａnd testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Subcellular Location : Membrane; Single-pass type I membrane protein.Tissue Specificity : Selectively expressed on hematopoietic progenitor cells ａnd the small vessel endothelium of a variety of tissues.Similarity : Belongs to the CD34 family.造血干细胞标志物内皮标志物 肿瘤生物标志物 细胞表面的唾液粘蛋白。产品图片Tissue/cell: rat bile duct; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-CD34 Polyclonal Antibody, Unconjugated（bs-0646R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingTissue/cell: human gastric adenocarcinoma;4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-CD34 Polyclonal Antibody, Unconjugated（bs-2038R） 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated（bs-0295G-Cy3）used at 1:200 dilution for 40 minutes at 37°C. Cell: rat Endothelial progenitor cells; Incubation: Anti-CD34 Polyclonal Antibody, PE conjugated（bs-2038R-PE） 1:50; Anti-CD133 Polyclonal Antibody, FITC conjugated（bs-4770R-FITC） 1:50 ; 40 minutes at 37°C. Blank control: Rabbit IgG/FITC（bsF-0295P） at 1:50 ａnd Rabbit IgG/PE（bs-0295P-PE） at 1:50