本公司经销C-jun/AP-1，原癌基因蛋白/活化蛋白1抗体，克隆类型为polyclonal，宿主来源是Rabbit，C-jun/AP-1原癌基因蛋白/活化蛋白1抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0670R
英文名称：Anti-C-jun/AP-1
中文名称：原癌基因蛋白/活化蛋白1抗体
其他名称：Jun oncogene; JUN; Activator Protein 1; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 36kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo, cow, shp, dog, chk, pig
产品介绍：The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, ａnd it encodes a protein which is highly homologous to the viral protein. cJun （previously known as the Fos binding protein p39） ａnd c Fos form a complex in the nucleus. AP 1 （activating protein 1） is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression ａnd function of cell cycle regulators such as Cyclin D1, p53, p21 （cip1/waf1）, p19 （ARF） ａnd p16. Fos ａnd Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations ａnd deletions in human malignancies.Function : Transcription factor that recognizes ａnd binds to the enhancer heptamer motif 5＇-TGA[CG]TCA-3＇. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.Subunit : Heterodimer with either FOS or BATF3 or ATF7. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA （By similarity）. Interacts with HIVEP3 ａnd MYBBP1A （By similarity）. Interacts with SP1, SPIB ａnd TCF20. Interacts with COPS5; the interaction leads indirectly to its phosphorylation. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts （via its basic DNA binding ａnd leucine zipper domains） with SMAD3 （via an N-terminal domain）; the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex. Interacts with RNF187. Binds to HIPK3.Subcellular Location : Nucleus.Post-translational modifications : Phosphorylated by CaMK4 ａnd PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3.[PTM] Phosphorylated at Thr-239, Ser-243 ａnd Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 ａnd Thr-286 thereby promoting JUN-mediated cell proliferation andtransformation.Similarity : Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP domain.转录调节因子（Transcriptin Regulators） C-jun（Oncoprotein C-jun：active protein 1）基因与鸟类肉瘤病毒17的转化基因具有同源性，是早期应答基因家族成员。主要用于各种恶性肿瘤的研究。C-jun又称应激活化蛋白激酶.产品图片Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-C-Jun Polyclonal Antibody, Unconjugated（bs-0670R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingProtein: line1, rat liver lysates, 30ug; line2, rat brain lysates, 30ug; Primary: Anti-C-jun/AP-1 （bs-0670R） at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bs-0295G-HRP） at 1: 3000; ECL excitated the fluorescence; Predicted band size : 36kDObserved band size : 36kD