本公司经销ChAT，ChAT胆碱乙酰转移酶抗体，克隆类型为polyclonal，宿主来源是Rabbit，ChATChAT胆碱乙酰转移酶抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0042R
英文名称：Anti-ChAT
中文名称：ChAT胆碱乙酰转移酶抗体
其他名称：Choline O acetyltransferase; Choline O acetyltransferase; Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 68-70kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo, dog, pig
产品介绍：The neurotransmitter acetycholine is synthesized from choline ａnd acetyl-coA by the choline acetyltransferase （ChAT）enzyme,and a specific marker of cholinergic neurons.The regulation of ChATactivity is thought to be important during the development of nervous systems interactions .The reduction of ChAT activity has been found in several neurodegenerative disorders including Alzheimer’s ａnd amyotrophic lateral sclerosis.Function : Catalyzes the reversible synthesis of acetylcholine （ACh） from acetyl CoA ａnd choline at cholinergic synapses.DISEASE : Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea （CMSEA） [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 （FIMG2）. CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, ａnd abrupt episodic crises with increased weakness, bulbar paralysis, ａnd apnea precipitated by undue exertion, fever, or excitement.Similarity : Belongs to the carnitine/choline acetyltransferase family.胆碱乙酰转移酶（Choline Acetyltransferase，ChAT 又称为：choline O-Acetyltransferase ,CHOACTase;）是催化胆碱和乙酰辅酶A转变为乙酰和辅酶A的一种酶。ChAT有M,S,R三个亚型，此抗体与三个亚型都反应。产品图片Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-ChAT Polyclonal Antibody, Unconjugated（bs-0042R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） stainingAntigen: bs-0042P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bs-0295G-HRP） at 1: 5000; TMB（C-0024） staining; Read the data in MicroplateReader by 450nm.