本公司经销Phospho-CHK2 （ser33），磷酸化细胞周期检测点激酶2抗体，克隆类型为polyclonal，宿主来源是Rabbit，Phospho-CHK2 （ser33）磷酸化细胞周期检测点激酶2抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-5260R
英文名称：Anti-Phospho-CHK2 （ser33）
中文名称：磷酸化细胞周期检测点激酶2抗体
其他名称：CDS 1; CDS1; Checkpoint kinase 2; Checkpoint like protein CHK2; Chek 2; Chek2; Chk 2; CHK2 checkpoint homolog （S. pombe）; CHK2 checkpoint homolog; HuCds 1; HuCds1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 60kDa
纯化方法：affinity purified by Protein A
交叉反应：hu
产品介绍：Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, ａnd may also participate in transduction of the DNA damage ａnd replicational stress signals. Chk2 is the mammalian ortholog of the budding yeast Rad53 ａnd fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine ａnd threonine residues （Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 ａnd Thr68） followed by glutamine （SQ or TQ motif）. These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation （IR）, UV irradiation or hydroxyurea treatment, Thr68 ａnd other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 ａnd Thr387 in the activation loop of the kinase domain. Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation ａnd the protein is rapidly phosphorylated in response to DNA damage ａnd to replication block.Subunit : "Homodimer. Homodimerization is part of the activation process but the dimer may dissociate following activation. Interacts with PML. Interacts with TP53. Interacts with RB1; phosphorylates RB1. Interacts with BRCA1. Interacts （phosphorylated at Thr-68） with MDC1; requires ATM-mediated phosphorylation of CHEK2. Interacts with TP53BP1; modulates CHEK2 phosphorylation at Thr-68 in response to ionizing radiation. Interacts with CDC25A; phosphorylates CDC25A ａnd mediates its degradation in response to ionizing radiation. Interacts with CUL1; mediates CHEK2 ubiquitination ａnd regulation.Subcellular Location : Nucleus.Tissue Specificity : High expression is found in testis, spleen, colon ａnd peripheral blood leukocytes. Low expression is found in other tissues.Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.Contains 1 FHA domain.Contains 1 protein kinase domain.Chk2作为Cdks的调节参与细胞周期调节过程,是生物进化过程中非常保守的蛋白激酶，在DNA损伤引起的细胞周期检测点调节中有着非常重要的作用。