Protein disulfide isomerase facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings. Protein disulfide Isomerase is expressed as soluble form in E.coli.

Unit definition: One unit causes a change in A650 of 0.01 per min of a 1.0 mg/ml solution of insulin the presence of dithiothreitol at pH 7.5 at 25 ^oC.

Purity: ≥ 95% on HPLC.

M.W.: 57119

Ref.: Gehting, M.J. and Sambrook, J. 1992. Nature 355, 33-45

Hilson, D.A, et al. 1984. Meth. Enzymol. 107, 281