T4 DNA ligase catalyzes the formation of phosphodiester bonds between double-stranded DNA strands with 3′ hydroxyl ａnd 5′ phosphate termini in the presence of ATP. ExpressLink T4 DNA Ligase formulation is optimized for faster reaction times ａnd more convenient incubation temperature than our other T4 DNA Ligase formulations.
Source: E. coli expressing a T4 DNA ligase gene.
Important benefits of ExpressLink? T4 DNA Ligase:
 Fast, 5 minute ligation of blunt- or cohesive-end DNA fragments.
 Ligation of PCR fragments with “A" overhangs in 15 minutes.
 Convenient 25° C reaction temperature eliminates the need for a water bath or temperature block.
 Ideal for cloning DNA into vectors, recircularization of linear DNA, library construction, TA cloning, ａnd linker ligation.
 Superior exonuclease quality control assay for exonuclease-free ligations
See the Difference the Cleanest Ligase Makes
We apply the most stringent test available for exonuclease contamination to every lot of ExpressLink T4 DNA Ligase. The superior sensitivity of our assay will detect much lower active exonuclease concentrations than assays used by other manufacturers. As a result, we offer one of the best ligase products on the market (see figure). You can be confident of achieving the highest quality data in your important experiments with ExpressLink? T4 DNA Ligase.
Performance ａnd Quality Testing: Endodeoxyribonuclease, 3′ ａnd 5′ exodeoxyribonuclease assays; ligation efficiency tested.
Unit Definition: One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37° C (One unit is equal to approximately 300 cohesive-end ligation units).

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