Description: This Human IL-1 beta Ready-SET-Go! (2nd Generation) ELISA Set contains the necessary reagents, standards, buffers ａnd diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate ａnd precise measurement protein levels from samples including serum, plasma, ａnd supernatants from cell cultures. This ELISA will recognize the cleaved mature form ａnd uncleaved pro-form of Human IL-1 beta. This second generation kit has increased sensitivity with a range from 2-150 pg/mL.

IL-1 beta is a proinflammatory cytokine expressed by monocytes, macrophages, ａnd dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, ａnd other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway ａnd is instead secreted alongside caspase-1 via an alternate ａnd incompletely understood mechanism. IL-1 beta signals through two receptors, IL-1RI ａnd IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells ａnd initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-1RA or IL-1 beta neutralizing antibodies potential therapeutical value.

Reactivity	Human
Sensitivity	2 pg/mL
Standard Curve Range	2 - 150 pg/mL
Components	Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant protein for generating standard curve ａnd calibrating samples 10X Coating Buffer . Buffer for plating the Capture Antibody 5X ELISA/ELISPOT Diluent . Buffer for blocking ａnd diluting the Detection Antibody ａnd Enzyme Enzyme. Pre-titrated Avidin-HRP Substrate. 1X TMB Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies ａnd standards 96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -76, -86)
Reported Applications	ELISA