Corning® Fibronectin, Human, 1mg (Product #354008)

Corning® Fibronectin, human, 1 mg, is used as a thin coating on tissue-culture surfaces to promote attachment, spreading, ａnd proliferation of a variety of cell types. The principal functions of fibronectin appear to be in cellular migration during wound healing ａnd development, regulation of cell growth ａnd differentiation, ａnd haemostasis/thrombosis.

Quality
Purity = 90% by SDS-PAGE
Tested for ability to promote attachment ａnd spreading of BHK-21 cells
Found negative for bacteria, fungi, ａnd mycoplasma

Source
Human plasma.Source material tested for hepatitis B antigen ａnd HIV-1 antibody.

Molecular Weight
440 kD (unreduced form)

Preparation ａnd Storage
Stable for three months at 2° to 8°C (lyophilized) or two weeks at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze-thaws

Handling
Recommended concentration - 1-5 µg/cm2 of growth surface for coating or at 5 µg/ml as a media additive

Formulation
Lyophilized from CAPS buffer containing NaCl ａnd CaCl2, pH 11.0